Diagnosing dry eye disease can be difficult because it largely depends on patient-reported symptoms and standard tests, often lacking accuracy. To improve this process, a new multimodal imaging system has been develop...
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ISBN:
(纸本)9781510683662;9781510683679
Diagnosing dry eye disease can be difficult because it largely depends on patient-reported symptoms and standard tests, often lacking accuracy. To improve this process, a new multimodal imaging system has been developed that combines swept-source optical coherence microscopy with confocal fluorescence microscopy. This system allows for the in vivo characterization and quantification of the biochemical composition of the ocular surface. In this proof-of-concept study, mouse models of evaporative dry eye disease (EDED) were tested using a fluorescently labeled ligand, specifically fluorescently labeled jacalin, which is a galactose-binding protein. The ligand was applied to the ocular surface, and after the animals were euthanized, the corneas were extracted, mounted, and imaged using a custom-built multimodal imaging system. This process accurately identified the binding sites of jacalin, demonstrating the system's potential to enhance the diagnosis and evaluation of therapies for evaporative EDED.
Light-evoked functional retinal imaging is of great interest in clinical and experimental ophthalmology because the alterations in retinal function hold the promise of being potentially more sensitive for disease diag...
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ISBN:
(纸本)9781510658257;9781510658264
Light-evoked functional retinal imaging is of great interest in clinical and experimental ophthalmology because the alterations in retinal function hold the promise of being potentially more sensitive for disease diagnosis than purely morphology-based assays. Recent progress in Optoretinography (ORG), a technique employing OCT to extract light-evoked functional response of retinal tissue, promises to provide the needed sensitivity to probe early alterations in retinal function. The most promising implementations of the ORG probing of photoreceptor function in clinical settings are using parallel OCT detection schemes such as Line Field and Full Field designs. Herein, we present the instrumentation scheme of a Full-Field-Swept-Source OCT (FF-SS-OCT) system incorporating a high-precision light stimulation channel for facilitating phase-based functional retinal imaging in mice. The assessment of the OCT signal phase errors and their correction using short-time Fourier transform (STFT) is detailed. The performance of the system is investigated using a model eye.
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