Cell mechanics studied by a reconstituted model tissue

作     者：Wakatsuki, T Kolodney, MS Zahalak, GI Elson, EL 

作者机构：Washington Univ Sch Med Dept Biochem & Mol Biophys St Louis MO 63110 USA Univ Calif Los Angeles Sch Med MacDonald Res Lab 1519 Dept MedDiv Dermatol Los Angeles CA 90095 USA Washington Univ Sch Engn Dept Biomed Engn St Louis MO 63130 USA Washington Univ Sch Engn Dept Mech Engn St Louis MO 63130 USA 

出 版 物：《BIOPHYSICAL JOURNAL》 (生物物理学杂志)

年 卷 期：2000年第79卷第5期

页      面：2353-2368页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 

基　　金：NIGMS NIH HHS [GM38838] Funding Source: Medline 

主　　题：生物力学 细胞计数 细胞 培养的 结缔组织细胞/生理学 细胞骨架/生理学 弹性 细胞外基质/生理学 成纤维细胞/生理学 模型 生物学 动物 鸡胚 

摘      要：Tissue models reconstituted from cells and extracellular matrix (ECM) simulate natural tissues. Cytoskeletal and matrix proteins govern the force exerted by a tissue and its stiffness. Cells regulate cytoskeletal structure and remodel ECM to produce mechanical changes during tissue development and wound healing. Characterization and control of mechanical properties of reconstituted tissues are essential for tissue engineering applications. We have quantitatively characterized mechanical properties of connective tissue models, fibroblast-populated matrices (FPMs), via uniaxial stretch measurements. FPMs resemble natural tissues in their exponential dependence of stress on strain and linear dependence of stiffness on force at a given strain. Activating cellular contractile forces by calf serum and disrupting F-actin by cytochalasin D yield active and passive components, which respectively emphasize cellular and matrix mechanical contributions. The strain-dependent stress and elastic modulus of the active component were independent of cell density above a threshold density. The same quantities for the passive component increased with cell number due to compression and reorganization of the matrix by the cells.