Soluble neuropilin-1 in gingival crevicular fluid is associated with rheumatoid arthritis: An exploratory case-control study

作     者：Prieto, Diego González, Camila Weber, Laura Realini, Ornella Pino-Lagos, Karina Bendek, Maria José Retamal, Ignacio Beltrán, Víctor Riedemann, Juan Pablo Espinoza, Francisco Chaparro, Alejandra 

作者机构：Department of Periodontology Center for Biomedical and Innovation Research Laboratory of Periodontal Research Faculty of Dentistry Universidad de los Andes Santiago Chile Centro de Investigación e Innovación Biomédica Facultad de Medicina Universidad de los Andes Santiago Chile Centre of Investigation and Innovation in Clinical Dentistry Faculty of Dentistry Universidad de la Frontera Temuco Chile Rheumatology Unit Faculty of Medicine Universidad de la Frontera Temuco Chile Department of Rheumatology Faculty of Medicine Universidad de los Andes Santiago Chile 

出 版 物：《Journal of Oral Biology and Craniofacial Research》 (J. Oral Biol. Craniofac. Res.)

年 卷 期：2021年第11卷第2期

页      面：303-307页

学科分类：1003[医学-口腔医学] 1002[医学-临床医学] 10[医学] 

基　　金：Universidad de La Frontera, UFRO Universidad de los Andes, Uniandes 

主　　题：Gingival crevicular fluid Neuropilin-1 Periodontitis Rheumatoid arthritis 

摘      要：Background: To explore the soluble Neuropilin-1 (sNRP-1) concentrations in gingival crevicular fluid (GCF) and the periodontal clinical status of patients with Rheumatoid Arthritis (RA). Materials and methods: We conducted an exploratory study with 40 study participants, 20 with RA, and 20 healthy controls. Clinical and periodontal data were recorded, and GCF samples were obtained. sNRP-1 levels in GCF were determined by ELISA assay. Descriptive statistics, Mann–Whitney U test, Unpaired t-test, logistic regression model, and Area Under Receiver Operating Characteristic Curve (AUC-ROC) were made to explore the diagnostic performance accuracy. Results: RA patients had significantly higher levels of sNRP-1 in GCF (p ​= ​0.0447). The median levels of GCF-sNRP-1 were 208.85 ​pg/μl (IQR 131.03) in the RA group compared to 81.46 ​pg/μl (IQR 163.73) in the control group. We observed an association between the GCF-sNRP-1 concentrations and the RA diagnosis (OR:1.009;CI 1.00–1.001;p ​= ​0.047). The diagnosis of chronic periodontitis was also associated with RA (OR: 6.9;CI 1.52–31.37;p ​= ​0.012). Moreover, the AUC-ROC of GCF-sNRP-1 concentrations combined with periodontal clinical parameters such as periodontal probing depth and periodontal inflamed surface area was 0.80. Conclusion: This exploratory case-control study shows that RA patients had significantly higher levels of sNRP-1 in GCF. New longitudinal studies are necessary to evaluate the role of NRP-1 in periodontal tissues and consider it an oral biomarker with clinical value in RA. © 2021 Craniofacial Research Foundation