Bacillus tequilensis Isolated from Fermented Intestine of Holothuria Scabra Produces Fibrinolytic Protease with Thrombolysis Activity

作     者：Nur Hidayati Nurrahman Nurrahman Hayatun Fuad Hendra Munandar Dewi Seswita Zilda Aditya Rahman Ernanto Amin Samiasih Oedjijono Oedjijono Stalis Norma Ethica 

作者机构：Magister Study Program of Medical Laboratory Science Universitas Muhammadiyah Semarang 50273 Indonesia Food Technology Study Program Faculty of Nursing and Health Sciences Universitas Muhammadiyah Semarang 50273 Indonesia Balai Bio Industri Laut - LIPI (Lembaga Ilmu Penelitian Indonesia) Lombok Nusa Tenggara Barat 83352 Indonesia Research and Development Center for Marine and Fishery Product Processing and Biotechnology Ministry of Maritime Affairs and Fisheries of Indonesia Jakarta 10260 Indonesia Faculty of Nursing and Health Sciences Universitas Muhammadiyah Semarang 50273 Indonesia Faculty of Biology Universitas Jenderal Soedirman Purwokerto 53122 Indonesia 

出 版 物：《IOP Conference Series: Earth and Environmental Science》 

年 卷 期：2021年第707卷第1期

摘      要：Among essential treatment of cardiovascular disorders are fibrinolytic proteases. Most thrombolysis agents are fibrinolytic enzymes from group of bacterial proteases. This work reports a potent bacterium isolated from fermented intestine of H. scabra, which could produce fibrinolytic protease with high thrombolysis activity. Bacterial selection was conducted based on proteolytic and fibrinolytic activities indicated as clear zone on skim milk and fibrin agar media, respectively. Crude proteases from the selected bacterial isolates were subjected to thrombolytic activity test based on gravimetric method, which results were confirmed after 7 repetitions. As result, 4 fibrinolytic protease-producing bacterial isolates HFSI-3, HFSI-4, HFSI-5 and HFSI-8 were obtained. Among them, HFSI-5 isolate identified as Bacillus tequilensis on the basis of the 16S rRNA gene sequencing and morphological properties produced crude protease with the highest thrombolytic activity. The thrombolytic activity of crude protease produced by B. tequilensis HFSI-5 is worthy of comparing to that of standard fibrinolytic enzyme Nattokinase showing its potential as thrombolysis agent.