KDM6B promotes ESCC cell proliferation and metastasis by facilitating C/EBPβ transcription

作     者：Qin, Mei Han, Fei Wu, Jian Gao, Feng-Xia Li, Yuan Yan, De-Xin He, Xue-Mei Long, Yang Tang, Xiao-Ping Ren, De-Lian Gao, Yan Dai, Tian-Yang 

作者机构：South West Med Univ Basic Med Coll Dept Immunol Luzhou Sichuan Peoples R China Med Univ Affiliated Hosp Southwest Dept Thorac Surg Luzhou Sichuan Peoples R China Southwest Med Univ Expt Med Ctr Affiliated Hosp Luzhou Sichuan Peoples R China 

出 版 物：《BMC CANCER》 (BMC癌症)

年 卷 期：2021年第21卷第1期

页      面：559-559页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：Youth Foundation of the Affiliated Hospital of South West Medical University Sichuan Science and Technology Plan projects [2016RZ0076] 

主　　题：ESCC KDM6B H3K27me3 C EBP beta 

摘      要：BackgroundAs an H3K27me3 demethylase and counteracts polycomb-mediated transcription repression, KDM6B has been implicated in the development and malignant progression in various types of cancers. However, its potential roles in esophageal squamous cell carcinoma (ESCC) have not been *** expression of KDM6B in human ESCC tissues and cell lines was examined using RT-qPCR, immunohistochemical staining and immunoblotting. The effects of KDM6B on the proliferation and metastasis of ESCC were examined using in vitro and in vivo functional tests. RNA-seq and ChIP-seq assay were used to demonstrate the molecular biological mechanism of KDM6B in *** show that the expression level of KDM6B increased significantly in patients with lymph node metastasis. Furthermore, we confirmed that KDM6B knockdown reduces proliferation and metastasis of ESCC cells, while KDM6B overexpression has the opposite effects. Mechanistically, KDM6B regulates TNFA_SIGNALING_VIA_NF kappa B signalling pathways, and H3K27me3 binds to the promoter region of C/EBP beta, leading to the promotion of C/EBP beta transcription. Besides, we show that GSK-J4, a chemical inhibitor of KDM6B, markedly inhibits proliferation and metastasis of ESCC *** present study demonstrated that KDM6B promotes ESCC progression by increasing the transcriptional activity of C/EBP beta depending on its H3K27 demethylase activity.