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Characterization, differentiation, and population doubling time of Wharton’s jelly mesenchymal stem cells (WJ-MSCs) in passage 5 and 8

作     者:Rizal Rahimi Syaidah Ziyan Muhammad Aqsha Adella Josephin Vidi Miranda Pakpahan 

作者机构:1Biomedical Engineering program Department of Electrical Engineering Faculty of Engineering Universitas Indonesia Kampus UI Depok West Java 16424 Indonesia 2Department of Histology Faculty of Medicine Universitas Indonesia Kampus UI Depok West Java 16424 Indonesia 3Undergraduate Program in Biomedical Engineering Department of Electrical Engineering Faculty of Engineering Universitas Indonesia Kampus UI Depok West Java 16424 Indonesia 

出 版 物:《AIP Conference Proceedings》 

年 卷 期:2021年第2344卷第1期

学科分类:07[理学] 0702[理学-物理学] 

摘      要:Wharton’s jelly mesenchymal stem cells (WJ-MSCs) is proposed to be the best source of mesenchymal stem cells (MSCs). It has better self-renewal and differentiation capacity compare to adult stem cells such as bone marrow- derived stem cells (BM-MSCs). However, their characteristics in different passages do not yet well described. This research aims to isolate and characterize WJ-MSCs with regard to their proliferation capacity, immunophenotypes, and differentiation capacity. The WJ-MSCs were isolated by the explant method. The cultured WJ-MSCs at P5 and P8 were then characterized using flow cytometric analysis with regard to its surface marker, which are CD44, CD90, CD105, CD73, and negative lineages. The proliferation capacity of WJ-MSCs was analyzed with the population doubling time method and the characterized cells were differentiated into three different lineages. The result revealed that the isolated cells highly express MSCs surface marker with 98% for all surface markers and 0.00% for negative lineages. Isolated WJ-MSCs also exhibited a high proliferation capacity with a range of 1.06 to 1.59 days. Both passages revealed excellent differentiation capacity into chondrocytes, adipocytes, and osteocytes.

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