Single-cell yolk-shell nanoencapsulation for long-term viability with size-dependent permeability and molecular recognition

作     者：Li Wang Yu Li Xiao-Yu Yang Bo-Bo Zhang N(o)elle Ninane Henk J.Busscher Zhi-Yi Hu Cyrille Delneuville Nan Jiang Hao Xie Gustaaf Van Tendeloo Tawfique Hasan Bao-Lian Su Li Wang;Yu Li;Xiao-Yu Yang;Bo-Bo Zhang;N(o)elle Ninane;Henk J.Busscher;Zhi-Yi Hu;Cyrille Delneuville;Nan Jiang;Hao Xie;Gustaaf Van Tendeloo;Tawfique Hasan;Bao-Lian Su

作者机构：State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China Laboratory of Inorganic Materials Chemistry (CMI) University of Namur Namur B-5000 Belgium State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China Laboratory of Inorganic Materials Chemistry (CMI) University of Namur Namur B-5000 Belgium Namur Research Institute for Life Sciences (Narilis) University of Namur Namur B-5000 Belgium Department of Biomedical Engineering University of Groningen and University Medical Centre Groningen Groningen 9713 AV The Netherlands State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China Nanostructure Research Centre (NRC) Wuhan University of Technology Wuhan 430070 China State Key Laboratory of Advanced Technology for Materials Synthesis and Processing Wuhan University of Technology Wuhan 430070 China School of Engineering and Applied Sciences Harvard University Cambridge MA 02138 USA Nanostructure Research Centre (NRC) Wuhan University of Technology Wuhan 430070 China Electron Microscopy for Materials Science (EMAT) University of Antwerp Antwerp B-2020 Belgium Cambridge Graphene Centre University of Cambridge Cambridge CB3 0FA UK 

出 版 物：《国家科学评论（英文版）》 (National Science Review)

年 卷 期：2021年第8卷第4期

页      面：120-136页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：国家自然科学基金 the International S&T Cooperation Projects of China 国家教育部长江学者与创新团队发展计划 湖北省自然科学基金 ‘Algae Factory' European H2020 Program financed by European Regional Development Fund and Wallonia Region of Belgium 国家留学基金委员会(CSC)项目 

主　　题：cell surface engineering protein internalization ordered colloidal packing high photosynthetic ability harsh condition resistance thiol-functionalization 

摘      要：Like nanomaterials,bacteria have been unknowingly used for *** hold significant economic potential for fuel and medicinal compound *** full exploitation,however,is impeded by low biological activity and stability in industrial *** cellular encapsulation addresses these limitations,cell survival is usually compromised due to shell-to-cell contacts and low ***,we report ordered packing of silica nanocolloids with organized,uniform and tunable nanoporosities for single cyanobacterium nanoencapsulation using protamine as an electrostatic template.A space between the capsule shell and the cell is created by controlled internalization ofprotamine,resulting in a highly ordered porous shell-void-cell structure *** unique yolk-shell nanostructures provide long-term cell viability with superior photosynthetic activities and resistance in harsh *** addition,engineering the colloidal packing allows tunable shell-pore diameter for size-dependent permeability and introduction of new functionalities for specific molecular *** strategy could significantly enhance the activity and stability of cyanobacteria for various nanobiotechnological applications.