What have we known so far for fluorescence staining and quantification of microplastics:A tutorial review

作     者：Shengdong Liu Enxiang Shang Jingnan Liu Yining Wang Nanthi Bolan MBKirkham Yang Li Shengdong Liu;Enxiang Shang;Jingnan Liu;Yining Wang;Nanthi Bolan;M.B.Kirkham;Yang Li

作者机构：Key Laboratory of Water and Sediment Sciences of Ministry of EducationState Key Laboratory of Water Environment SimulationSchool of EnvironmentBeijing Normal UniversityBeijing 100875China College of Science and TechnologyHebei Agricultural UniversityHuanghua 061100China School of Agriculture and EnvironmentThe University of Western AustraliaPerthWA 6001Australia The UWA Institute of AgricultureThe University of Western AustraliaPerthWA 6001Australia Global Innovative Centre for Advanced NanomaterialsCollege of EngineeringScience and EnvironmentUniversity of NewcastleCallaghanNSW 2308Australia Department of AgronomyThrockmorton Plant Sciences CenterKansas State UniversityManhattanKS 66506USA 

出 版 物：《Frontiers of Environmental Science & Engineering》 (环境科学与工程前沿（英文）)

年 卷 期：2022年第16卷第1期

页      面：37-50页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：This study was supported by the National Key R&D Program of China(Grant No.2017YFA0605001) the National Natural Science Foundation of China(Grant Nos.52170024,21677015 and 22006031) the Natural Science Foundation of Hebei Province(No.B2019204315) the Sponsored Research Overhead Fund(Grant No.472120)from Kansas State University 

主　　题：Plastic particles Fluorescence dyes Identification Concentration quantification 

摘      要：Understanding the fate and toxicity of microplastics(MPs,5 mm plastic particles)is limited by quantification *** paper summarizes the methods in use and presents new ***,sampling and pretreatment processes ofMPs,including sample collection,digestion,density separation,and quality control are *** the promising and convenient staining procedures and quantification methods for MPs using fluorescence dyes are *** factors that influence the staining of MPs,including their physicochemical properties,are summarized to provide an optimal operation *** general,the digestion step is crucial to eliminate natural organic matter(NOM)to avoid interference in *** was reported to be the most appropriate solvent,and 10–20μg/mL are recommended as optimal dye *** addition,a heating and cooling procedure is recommended to maintain the fluorescence intensity of MPs for two *** staining,a fluorescence microscope is usually used to characterize the morphology,mass,or number of MPs,but compositional analysis cannot be determined with *** fluorescence staining methods have been implemented to study MP abundance,transport,and toxicity and have been combined with other chemical characterization techniques,such as Fourier transform infrared spectroscopy and Raman *** studies are needed to focus on the synthesis of novel dyes to avoid NOM’s *** need to be combined with other spectroscopic techniques to characterize plastic composition and to develop image-analysis *** stability of stained MPs needs to be improved.