Rational Design of a Negative Photochromic Spiropyran-Contained Fluorescent Polymeric Nanoprobe for Sulfur Dioxide Ratiometric Detection and in Vivo Imaging

作     者：Chen, Jian Li, Xiang Lin, Zhong Tian, Yong Zhang, Chonghua Zhang, Peisheng Zeng, Rongjin Qiao, Lei 

作者机构：Key Laboratory of Theoretical Organic Chemistry and Functional Molecule of Ministry of Education Hunan Provincial Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers Hunan Provincial Key Lab of Advanced Materials for New Energy Storage and Conversion Hunan Province College Key Laboratory of QSAR/QSPR School of Chemistry and Chemical Engineering Hunan University of Science and Technology Hunan Xiangtan411201 China Central Laboratory of the Affiliated Xuzhou Municipal Hospital of Xuzhou Medical University Xuzhou first People's Hospital Jiangsu Xuzhou221116 China Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science MOE Shandong Key Laboratory of Biochemical Analysis College of Chemistry and Molecular Engineering Qingdao University of Science and Technology China 

出 版 物：《SSRN》 

年 卷 期：2022年

核心收录：

主　　题：Addition reactions 

摘      要：It is highly desirable to develop high-performance of ratiometric fluorescent probes for SO2 detection and realize their application in biological imaging and food detection. In this study, we reported the rational design of a novel negative photochromic spiropyran derivative as Spiro[azahomoadamantane-pyran] (MAHD-SP) with notable orange fluorescence in its stable ring-opened state without UV regulation. The unsaturated double bond of MAHD-SP can undergo Michael addition reaction of SO2 , which makes the obviously fluorescence quenching of MAHD-SP. Then, MAHD-SP, a fluorescent conjugated polymer PFO and a polymeric surfactant PEO113 - b -PS49 were used to construct a ratiometric fluorescent polymeric nanoprobe ( RFPN ) via co-precipitation method. The probe exhibited high sensitivity and selectivity toward ratiometric detection of SO2 in pure aqueous solutions. Moreover, RFPN was applied to quantitative analysis of SO2 in food and water samples. The good biocompatibility of RFPN can also be used to visualization of exogenous and endogenous SO2 generation in zebrafish model. © 2022, The Authors. All rights reserved.