Hook, Line, and Sinker! Spectroscopic Studies of Bi-Modular Mono- and Bis-1,8-naphthalimide-Ru(bpy)3-conjugates as DNA "Light Switches"

作     者：Ryan, Gary J. Gunnlaugsson, Thorfinnur Quinn, Susan J. 

作者机构：Univ Dublin Trinity Biomed Sci Inst TBSI Trinity Coll Dublin Sch Chem Dublin 2 Ireland Synth & Solid State Pharmaceut Ctr SSPC Bernal Inst Limerick V94 T9PX Ireland Univ Coll Dublin Sch Chem Dublin 4 Ireland 

出 版 物：《INORGANIC CHEMISTRY》 (无机化学)

年 卷 期：2022年第61卷第31期

页      面：12073-12086页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0703[理学-化学] 070301[理学-无机化学] 

基　　金：Science Foundation Ireland [13/IA/1865, 2: 12/RC/2275_P2] European Regional Development Fund Irish Research Council Science Foundation Ireland (SFI) [13/IA/1865] Funding Source: Science Foundation Ireland (SFI) 

主　　题：Absorption Genetics Ligands Metal to ligand charge transfer Peptides and proteins 

摘      要：Bi-chromophoric ruthenium polypyridyl complexes comprising one or two nitro-1,8-naphthalimide groups are shown to be effective DNA binders with off-on light switching properties. The binding to DNA was investigated using a combination of studies such as UV-visible absorption and emission titrations, thermal denaturation, and circular dichroism spectroscopy. The DNA affinity was shown to be sensitive to both the linker length and the number of naphthalimides (one vs two) contained in these systems and binding constants ranging from 10(6) to 10(7) M-1 for salmon testes DNA. The strong DNA binding is attributed to the combination of naphthalimide intercalation and the electrostatic interaction of the ruthenium complex. Large emission enhancements from the metal to ligand charge transfer (MLCT) emission arising from the metal complex were observed upon DNA binding, which was attributed to the interruption of intramolecular electron transfer quenching processes. Moving the nitro substitution from the 4-position to the 3-position is found to result in modification of the DNA binding and the resulting optical properties. The off-on light switch phenomena reported demonstrate the potential of these complexes to act as DNA probes.