Modulation of dynamic aggregation in fluorogenic SNAP-tag probes for long-term super-resolution imaging

作     者：Qinglong Qiao Wenjuan Liu Weijie Chi Jie Chen Wei Zhou Ning Xu Jin Li Xiangning Fang Yi Tao Yinchan Zhang Yingzhu Chen Lu Miao Xiaogang Liu Zhaochao Xu 

作者机构：CAS Key Laboratory of Separation Science for Analytical ChemistryDalian Institute of Chemical PhysicsChinese Academy of SciencesDalianChina University of Chinese Academy of SciencesBeijingChina Fluorescence Research GroupSingapore University of Technology and DesignSingaporeSingapore 

出 版 物：《Aggregate》 (聚集体（英文）)

年 卷 期：2023年第4卷第2期

页      面：173-182页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:22078314,21878286,21908216 Dalian Institute of Chemical Physics,Grant/Award Numbers:DICPI202142,DICPI201938,DICPZZBS201805 Advanced Manufacturing and Engineering Program,Grant/Award Number:A2083c0051 Ministry of Education,Singapore,Grant/Award Number:MOE-MOET2EP10120-0007 

主　　题：aggregation dimer fluorogenic SNAP-tag super-resolution imaging 

摘      要：The combination of super-resolution microscopy and synthetic fluorescence probes has emerged as a universal tool to monitor dynamic biological events at the nanometer ***,the limited site-specificity and fluorogenicity of synthetic fluorescent probes make it still difficult to realize long-term super-resolution ***,we introduce a dynamic aggregation mediated SNAP-tag fluorogenic probe,BGAN-Aze,which can specifically bind to various SNAP-tag fusion proteins with 41-fold fluorescence *** equilibrium between the non-fluorescent aggregate/dimer(A–D)and the fluorescent monomer(M)of BGAN-Aze acts as an effective method to reduce the fluorescence background and endow BGAN-Aze with the capability of conducting washing-free super-resolution imaging of various intracellular and extracellular *** this probe,we monitored multiple dynamic biological events,such as MMC,mitophagy,the fusion of nucleolus,and the growth and contact of *** expect that BGAN-Aze will become a widely used SNAP-tag for super-resolution imaging of dynamic biological events and the A-D-M equilibrium can be a general strategy for designing fluorogenic probes.