Inhibition of Cancer Cell Migration and Glycolysis by Terahertz Wave Modulation via Altered Chromatin Accessibility

作     者：Lan Suno Yangmei Li Yun Yu Peiliang Wang Shengquan Zhu KaijieWu Yan Liu Ruixing Wang Li Min Chao Chan Lan Sun;Yangmei Li;Yun Yu;Peiliang Wang;Shengquan Zhu;Kaijie Wu;Yan Liu;Ruixing Wang;Li Min;Chao Chang

作者机构：Innovation Laboratory of Terahertz BiophysicsNational Innovation Institute of Defense TechnologyBeijing 100071China School of Psychological and Cognitive SciencesPeking UniversityBeijing 100871China Aerospace Information Research InstituteSchool of ElectronicElectrical and Communication EngineeringUniversity of the Chinese Academy of SciencesBeijing 100049China Key Laboratory of Electromagnetic Illumination and Sensing TechnologyChinese Academy of SciencesBeijing 100190China Department of GastroenterologyBeijing Friendship HospitalCapital Medical UniversityBeijing 100050China Beijing Key Laboratory for Precancerous Lesion of Digestive DiseaseNational Clinical Research Center for Digestive DiseaseBeijing100171China School of PhysicsPeking UniversityBeijing 100871China 

出 版 物：《Research》 (研究（英文）)

年 卷 期：2022年第2022卷第4期

页      面：381-396页

核心收录：

学科分类：0809[工学-电子科学与技术（可授工学、理学学位）] 08[工学] 

基　　金：This work was financially supported by the China Postdoctoral Science Foundation(2020M673689) the National Natural Science Foundation of China(82073390,81702314,61905286,and T2241002) the Beijing Science and Technology Nova Program(Z191100001119128) the Beijing Municipal Science and Technology Project(Z191100006619081) the National Defense Technology Innovation Special Zone.CC acknowledges the support of X-plore Prize 

主　　题：drugs Together migration 

摘      要：Metastasis and metabolic disorders contribute to most cancer deaths and are potential drug targets in cancer treatment. However, corresponding drugs inevitably induce myeloid suppression and gastrointestinal toxicity. Here, we report a nonpharmaceutical and noninvasive electromagnetic intervention technique that exhibited long-term inhibition of cancer cells. Firstly, we revealed that optical radiation at the specific wavelength of 3.6 μm (i.e., 83 THz) significantly increased binding affinity between DNA and histone via molecular dynamics simulations, providing a theoretical possibility for THz modulation- (THM-) based cancer cell intervention. Subsequent cell functional assays demonstrated that low-power 3.6 μm THz wave could successfully inhibit cancer cell migration by 50% and reduce glycolysis by 60%. Then, mRNA sequencing and assays for transposase-accessible chromatin using sequencing (ATAC-seq) indicated that low-power THM at 3.6 μm suppressed the genes associated with glycolysis and migration by reducing the chromatin accessibility of certain gene loci. Furthermore, THM at 3.6 μm on HCT-116 cancer cells reduced the liver metastasis by 60% in a metastatic xenograft mouse model by splenic injection, successfully validated the inhibition of cancer cell migration by THM in vivo. Together, this work provides a new paradigm for electromagnetic irradiation-induced epigenetic changes and represents a theoretical basis for possible innovative therapeutic applications of THM as the future of cancer treatments.