Exercise regulates shelterin genes and microRNAs implicated in ageing in Thoroughbred horses

作     者：Mandal, Shama Denham, Michele M. Spencer, Sarah J. Denham, Joshua 

作者机构：RMIT Univ Sch Hlth & Biomed Sci Melbourne Vic Australia Jubilee Stud Freshwater Creek Vic Australia RMIT Univ ARC Ctr Excellence Nanoscale Biophoton Melbourne Vic Australia Univ Southern Queensland Sch Hlth & Med Sci Ipswich Qld 4305 Australia Univ New England Sch Sci & Technol Armidale NSW Australia 

出 版 物：《PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY》 (欧洲生理学杂志)

年 卷 期：2022年第474卷第11期

页      面：1159-1169页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071003[理学-生理学] 

基　　金：School of Science and Technology at the University of New England the School of Health and Biomedical Sciences at RMIT University 

主　　题：Telomere TERT Non-coding RNA miRNA Equine 

摘      要：Ageing causes a gradual deterioration of bodily functions and telomere degradation. Excessive telomere shortening leads to cellular senescence and decreases tissue vitality. Six proteins, called shelterin, protect telomere integrity and control telomere length through telomerase-dependent mechanisms. Exercise training appears to maintain telomeres in certain somatic cells, although the underlying molecular mechanisms are incompletely understood. Here, we examined the influence of a single bout of vigorous exercise training on leukocyte telomerase reverse transcriptase (TERT) and shelterin gene expression, and the abundance of three microRNAs (miRNAs) implicated in biological ageing (miRNA-143, -223 and -486-5p) in an elite athlete and large animal model, Thoroughbred horses. Gene and miRNA expression were analysed using primer-based and TaqMan Assay qPCR. Leukocyte TRF1, TRF2 and POT1 expression were all significantly increased whilst miR-223 and miR-486-5p were decreased immediately after vigorous exercise (all p 0.05), and tended to return to baseline levels 24 h after training. Relative to the young horses (similar to 3.9 years old), middle-aged horses (similar to 14.8 years old) exhibited reduced leukocyte TERT gene expression, and increased POT1 and miR-223 abundance (all p 0.05). These data demonstrate that genes transcribing key components of the shelterin-telomere complex are influenced by ageing and dynamically regulated by a single bout of vigorous exercise in a large, athletic mammal - Thoroughbred horses. Our findings also implicate TERT and shelterin gene transcripts as potential targets of miR-223 and miR-486-5p, which are modulated by exercise and may have a role in the telomere maintenance and genomic stability associated with long-term aerobic training.