Surface Modification of Pcl-Gelatin-Chitosan Nanofibrous Scaffold by Nanohydroxyapatite for Bone Tissue Engineering

作     者：Gautam, Sneh Purohit, Shiv Dutt Singh, Hemant Dinda, Amit Kumar Potdar, Pravin D. Sharma, Chhavi Chou, Chia-Fu Mishra, Narayan Chandra 

作者机构：Department of Molecular Biology & Genetic Engineering CBSH G. B. Pant University of Agriculture & Technology Pantnagar India School of Chemical Engineering Yeungnam University Gyeongsan38541 Korea Republic of Department of Polymer & Process Engineering Indian Institute of Technology Roorkee India Department of Pathology All India Institute of Medical Science New Delhi India Institute of Physics Academia Sinica Taipei Taiwan Department of Molecular Medicine & Biology Jaslok Hospital & Research Centre Mumbai India Avantha Centre for Industrial Research and Development Punjab Patiala India 

出 版 物：《SSRN》 

年 卷 期：2022年

核心收录：

主　　题：Chitosan 

摘      要：Bone tissue engineering (BTE) has attained enough attention towards fabricating a scaffold for the treatment of bone injury. To develop an ideal scaffold, various techniques and polymers have been explored. Electrospinning has been much focused on fabricating a scaffold by taking various polymers for bone tissue engineering in recent years. In this study, a tri-polymer (PCL-gelatin-chitosan;PCL-GT-CS) scaffold was first fabricated through electrospinning. Then, the surface of the fabricated scaffold was treated with 1 wt% of nanohydroxyapatite (nHAp) for 10, 20 and 30 minutes. After treatment, nHAp modified PCL-GT-CS nanofibrous scaffold was studied by field emission scanning electron microscopy (FE-SEM) to investigate the surface morphology, fiber diameter and pore size. FE-SEM analysis revealed a uniform distribution of nHAp particles on the surface of nanofibers in PCL-GT-CS scaffold after 20 minutes. The fiber diameter and pore size of nHAp treated scaffold (20 minutes) were determined and found to be 680 ± 184 nm and 5.3 ± 2.1 µm respectively. Further, X-ray diffraction (XRD), Energy dispersive X-ray spectroscopy (EDS), Fourier transform infrared (FT-IR) spectroscopy, thermogravimetric (TG) and derivative thermogravimetric analysis (DTG) suggested the presence of nHAp over the surface of PCL-GT-CS nanofibrous scaffold. The cell viability of human osteoblasts over the nHAp modified PCL-GT-CS scaffold was analyzed by MTT assay which was found higher than the unmodified (PCL-GT-CS) scaffold. Furthermore, DNA quantification was performed to examine the proliferation of osteoblasts which was also found significantly higher on nHAp modified scaffold than the unmodified scaffold. Moreover, FE-SEM analysis of the scaffold, seeded with cell, depicted effective cell attachment of osteoblasts, well-spread morphology with their characteristic polygonal shape over the modified PCL-GT-CS nanofibrous scaffold. Thus, in-vitro study of nHAp modified PCL-GT-CS nanofibrous scaffo