Sustainable bioconversion of agricultural waste substrates into poly (3-hydroxyhexanoate) (mcl-PHA) by Cupriavidus necator DSM 428

作     者：Razzaq, Sadia Shahid, Salma Farooq, Robina Noreen, Sadia Perveen, Sofia Bilal, Muhammad 

作者机构：Govt Coll Women Univ Faisalabad Dept Chem Faisalabad 38040 Pakistan Govt Coll Women Univ Faisalabad Dept Biochem Faisalabad 38040 Pakistan Huaiyin Inst Technol Sch Life Sci & Food Engn Huaian 223003 Peoples R China 

出 版 物：《BIOMASS CONVERSION AND BIOREFINERY》 (生物质转化与生物炼制)

年 卷 期：2024年第14卷第8期

页      面：9429-9439页

核心收录：

学科分类：0820[工学-石油与天然气工程] 081704[工学-应用化学] 07[理学] 0817[工学-化学工程与技术] 070304[理学-物理化学(含∶化学物理)] 08[工学] 0703[理学-化学] 

基　　金：Higher Education Commission (HEC), Islamabad, Pakistan Government College Women University, Faisalabad, Pakistan 

主　　题：Microorganisms Agricultural waste Polyester Polyhydroxyalkanoate C necator Molasses Olive oil 

摘      要：Polyhydroxyalkanoate (PHA) synthesised by microbial strains has properties (biocompatible, non-toxic, and biodegradable) that make it appropriate as an environment-friendly plastic component. This research evaluated the cheap carbon substrates (molasses, olive oil, and their mixture) of industrial waste as an alternative to costly ones for the manufacturing enhancement of mcl-PHA by C. necator. The strain was cultured in both nutrient and mineral media with and without nitrogen. mcl-PHA content was found to be 24.33%, 18.66%, and 40% with molasses, olive oil, and a mixture of both substrates, respectively. The chromatographic technique, GCMS, was utilized to confirm the types of PHA monomers (3HB and HHx). The maximum PHA content produced was 2.03 g/L using a combination of substrates compared to molasses (1.41 g/L) and olive oil (1.12 g/L). Morphology exhibits pseudo-spherical granules with comparatively consistent distribution by SEM. FTIR spectroscopy was used to detect PHA presence rapidly. In conclusion, C. necator DSM 428 cultivated on a mixture of substrates is proficient in manufacturing mcl-PHA along with scl-PHA;the type of PHA monomer depends upon the selection of substrate.