版权所有:内蒙古大学图书馆 技术提供:维普资讯• 智图
内蒙古自治区呼和浩特市赛罕区大学西街235号 邮编: 010021
作者机构:College of Biosystems Engineering and Food ScienceNational-Local Joint Engineering Laboratory of Intelligent Food Technology and EquipmentZhejiang Key Laboratory for Agro-Food ProcessingIntegrated Research Base of Southern Fruit and Vegetable Preservation TechnologyFuli Institute of Food ScienceZhejiang UniversityHangzhouChina College of Food Science and EngineeringHainan Tropical Ocean UniversityYazhou Bay Innovation InstituteMarine Food Engineering Technology Research Center of Hainan ProvinceCollaborative Innovation Center of Marine Food Deep ProcessingSanyaChina Department of Food Production and Environmental ManagementFaculty of AgricultureIwate UniversityUeda4-3-5MoriokaJapan
出 版 物:《Food Quality and Safety》 (食品品质与安全研究(英文版))
年 卷 期:2022年第6卷第3期
页 面:435-445页
核心收录:
学科分类:1004[医学-公共卫生与预防医学(可授医学、理学学位)] 100403[医学-营养与食品卫生学] 10[医学]
基 金:supported by the Hainan Provincial Natural Science Foundation of China (321CXTD1012) the National Natural Science Foundation of China (NSFC31871868) the Scientific Research Foundation of Hainan Tropical Ocean University (RHDRC202117),China
主 题:Shewanella putrefaciens citral quorum sensing system gene expression analysis
摘 要:Objectives:The bacteriostatic effects of a citral nanoemulsion against Shewanella putrefaciens CN-32(SHP CN-32)were investigated using in vitro culture and gene expression analysis,forbuilding a potential application in spoilage microorganism control and aquatic products quality *** and Methods:SHP CN-32 was treated by prepared citral nanoemulsion when the minimal inhibitory concentration(MIC)was *** growth curve,membrane integrity,scanning electron microscope(SEM)observation,biofilm formation and quorum sensing(QS)signaling molecule Al-2 content were evaluated in different MIC treatment groups(0 to 1.00 MIC).The gene expression status of SHP CN-32 in O and 0.50 MIC groups were compared using transcriptome sequencing and quantitative polymerase chain reaction(PCR).Results:The in vitro culture revealed that the citral nanoemulsion could inhibit the growth of SHP CN-32 with MIC of approximately 200μg/*** of membrane *** and biofilm formation suggested significant biological structure damage in bacteria after ***,the Qs signaling molecule Al-2 content showed a decline with increasing treatment *** sequencing and quantitative PCR revealed that the majority genes related diversified functional metabolic pathways of SHP CN-32 were downregulated at varying ***:A significant bacteriostasis of citral nanoemulsion against SHP CN-32 was verified via the results of growth inhibition,structural destruction,signal molecular decrease and gene expression downregulation of *** synergies significantly affect the characteristic expression of SHP CN-32,revealing the application potential as bacteriostat,QS inhibitor and preservative in aquatic products.