Evaluation of thermostable endoglucanase in Paenibacillus lautus strain BHU3 for yield enhancement

作     者：Suman Yadav Anand Kumar Pandey Suresh Kumar Dubey 

作者机构：Molecular Ecology LaboratoryCentre of Advanced Study in BotanyInstitute of ScienceBanaras Hindu UniversityVaranasi 221005India Department of Biotechnology EngineeringInstitute of Engineering and TechnologyBundelkhand UniversityJhansi 284128India 

出 版 物：《Systems Microbiology and Biomanufacturing》 (系统微生物学与生物制造（英文）)

年 卷 期：2022年第2卷第4期

页      面：607-622页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：This research work was financially assisted by University Grants Commission Government of India New Delhi in the form of Junior and Senior Research Fellowship to Suman(Grant No.09/013(0617)/2016-EMR-I) 

主　　题：Agrowastes Cellulose RAST Endoglucanase Paenibacillus lautus 

摘      要：In the present study,Paenibacillus lautus strain BHU3 isolated from landfill soil was evaluated for the presence of potential endoglucanases which are the first candidate of cellulase enzyme system to act on ***-silico analysis revealed high potential thermostable endoglucanases which can efficiently interact with *** most potent and thermostable endoglucanase(locus tag ***30_18280)belonged to glycosyl family-5 and had interaction energy of−12.981 kcal/mol for the best docked cluster containing three out of ten docking conformations,and Tm value of 73.3℃.MD simulation of 100 ns proved highly stable binding interactions of CPZ30_18280 endoglucanase with cellulose with root mean square deviation(RMSD)values ranging from 0.15 to 0.30 *** interactions with characteristic active site residues(tyrosine,tryptophan and aspartate)of glycosyl family-5 endoglucanases were ***,to enhance the production of endoglucanases,the fermentation conditions were optimized employing approaches like one factor at a time(OFAT)and response surface methodology(RSM).Maximum activity of endoglucanase was determined at 60℃.The optimized condi-tions for enhanced production of endoglucanase(10.15 U/mL)were pH 6.63,yeast extract conc.3.44 g/L,wheat bran 3.59%,and inoculum size 2.65%.Hence,*** strain BHU3 has enormous potential to synthesize highly efficient thermostable endoglucanases under optimized regime using ***,it could find immense industrial applications including large scale cellulose conversion to bioethanol.