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Evaluation of thermostable endoglucanase in Paenibacillus lautus strain BHU3 for yield enhancement

作     者:Suman Yadav Anand Kumar Pandey Suresh Kumar Dubey 

作者机构:Molecular Ecology LaboratoryCentre of Advanced Study in BotanyInstitute of ScienceBanaras Hindu UniversityVaranasi 221005India Department of Biotechnology EngineeringInstitute of Engineering and TechnologyBundelkhand UniversityJhansi 284128India 

出 版 物:《Systems Microbiology and Biomanufacturing》 (系统微生物学与生物制造(英文))

年 卷 期:2022年第2卷第4期

页      面:607-622页

核心收录:

学科分类:081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070303[理学-有机化学] 0703[理学-化学] 

基  金:This research work was financially assisted by University Grants Commission Government of India New Delhi in the form of Junior and Senior Research Fellowship to Suman(Grant No.09/013(0617)/2016-EMR-I) 

主  题:Agrowastes Cellulose RAST Endoglucanase Paenibacillus lautus 

摘      要:In the present study,Paenibacillus lautus strain BHU3 isolated from landfill soil was evaluated for the presence of potential endoglucanases which are the first candidate of cellulase enzyme system to act on ***-silico analysis revealed high potential thermostable endoglucanases which can efficiently interact with *** most potent and thermostable endoglucanase(locus tag ***30_18280)belonged to glycosyl family-5 and had interaction energy of−12.981 kcal/mol for the best docked cluster containing three out of ten docking conformations,and Tm value of 73.3℃.MD simulation of 100 ns proved highly stable binding interactions of CPZ30_18280 endoglucanase with cellulose with root mean square deviation(RMSD)values ranging from 0.15 to 0.30 *** interactions with characteristic active site residues(tyrosine,tryptophan and aspartate)of glycosyl family-5 endoglucanases were ***,to enhance the production of endoglucanases,the fermentation conditions were optimized employing approaches like one factor at a time(OFAT)and response surface methodology(RSM).Maximum activity of endoglucanase was determined at 60℃.The optimized condi-tions for enhanced production of endoglucanase(10.15 U/mL)were pH 6.63,yeast extract conc.3.44 g/L,wheat bran 3.59%,and inoculum size 2.65%.Hence,*** strain BHU3 has enormous potential to synthesize highly efficient thermostable endoglucanases under optimized regime using ***,it could find immense industrial applications including large scale cellulose conversion to bioethanol.

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