Ptychographic lens-less polarization microscopy

作     者：Kim, Jeongsoo Song, Seungri Kim, Bora Park, Mirae Oh, Seung Jae Kim, Daesuk Cense, Barry Huh, Yong-Min Lee, Joo Yong Joo, Chulmin 

作者机构：Department of Mechanical Engineering Yonsei University Seoul03722 Korea Republic of Department of Ophthalmology Asan Medical Center University of Ulsan College of Medicine Seoul05505 Korea Republic of Department of Radiology College of Medicine Yonsei University Seoul03722 Korea Republic of YUHS-KRIBB Medical Convergence Research Institute Seoul03722 Korea Republic of Department of Mechanical System Engineering Jeonbuk National University Jeonju54896 Korea Republic of Optical and Biomedical Engineering Laboratory Department of Electrical Electronic & Computer Engineering The University of Western Australia PerthWA6009 Australia Department of Biochemistry & Molecular Biology College of Medicine Yonsei University Seoul03722 Korea Republic of 

出 版 物：《arXiv》 (arXiv)

年 卷 期：2022年

核心收录：

主　　题：Birefringence 

摘      要：Birefringence, an inherent characteristic of optically anisotropic materials, is widely utilized in various imaging applications ranging from material characterizations to clinical diagnosis. Polarized light microscopy enables high-resolution, high-contrast imaging of optically anisotropic specimens, but it is associated with mechanical rotations of polarizer/analyzer and relatively complex optical designs. Here, we present a novel form of polarization-sensitive microscopy capable of birefringence imaging of transparent objects without an optical lens and any moving parts. Our method exploits an optical mask-modulated polarization image sensor and single-input-state LED illumination design to obtain complex and birefringence images of the object via ptychographic phase retrieval. Using a camera with a pixel resolution of 3.45 µm, the method achieves birefringence imaging with a half-pitch resolution of 2.46 µm over a 59.74 mm2 field-of-view, which corresponds to a space-bandwidth product of 9.9 megapixels. We demonstrate the high-resolution, large-area birefringence imaging capability of our method by presenting the birefringence images of various anisotropic objects, including a birefringent resolution target, liquid crystal polymer depolarizer, monosodium urate crystal, and excised mouse eye and heart tissues. © 2022, CC BY.