Metagenomic Insights into the Effects of Benzyl Dodecyl Dimethyl Ammonium Bromide (Bdab) Shock on Bacterial-Driven Nitrogen Removal in Moving Bed Biofilm Reactor (Mbbr)

作     者：Wang, Jing Li, Ling Chi, Baihui Shan, Jiajia Yi, Xianliang Liu, Yang Zhou, Hao 

作者机构：Key Laboratory of Industrial Ecology and Environmental Engineering Ministry of Education School of Ocean Science and Technology Dalian University of Technology Panjin Campus China 

出 版 物：《SSRN》 

年 卷 期：2022年

核心收录：

主　　题：Nitrogen removal 

摘      要：The usage of quaternary ammonium compounds (QACs) has greatly increased as disinfectants since the outbreak of SARs-CoV-2. However, the effect of QACs to the wastewater treatment performance was still unclear. In this study, a commonly used QACs, i.e., benzyl dodecyl dimethyl ammonium bromide (BDAB), was added into moving bed biofilm reactor (MBBR) to investigate its effect on nutrient removal. When the BDAB concentration increased to 50 mg L -1 , the ammonia removal efficiency (ARE) was greatly decreased, also for the ammonia degradation (ADR) and nitrate production (NPR) rates. This inhibition could partly be recovered by decreasing the BDAB concentration to 30 mg L -1 again. Metagenomic sequencing revealed the functional genera in different stages of control (Rc) and BDAB-added reactor (Re). The functional genes analysis suggested the lack of ammonia oxidase-encoding genes ( amo ABC) may cause the decrease of ARE in Re, while the efflux pump-encoding genes emr E, mdf A, and opr M and a gene encoding BAC oxygenase ( oxy BAC) were responsible for BDAB resistance. The increase of the total abundance of antibiotic resistant genes (ARGs) in Re revealed the potential risk caused by BDAB. Overall, this study revealed the potential effect and ecological risk of BDAB released into WWTPs. © 2022, The Authors. All rights reserved.