Transporter Engineering in Microbial Cell Factory Boosts Biomanufacturing Capacity

作     者：Xiaodong Lv Haijie Xue Lei Qin Chun Li 

作者机构：Key Laboratory of Medical Molecule Science and Pharmaceutics EngineeringMinistry of Industry and Information TechnologyInstitute of Biochemical EngineeringSchool of Chemistry and Chemical EngineeringBeijing Institute of TechnologyBeijingChina Key Lab for Industrial BiocatalysisMinistry of EducationDepartment of Chemical EngineeringTsinghua UniversityBeijingChina Center for Synthetic and Systems BiologyDepartment of Chemical EngineeringTsinghua UniversityBeijingChina 

出 版 物：《BioDesign Research》 (生物设计研究（英文）)

年 卷 期：2022年第2022卷第1期

页      面：228-235页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 071005[理学-微生物学] 10[医学] 

基　　金：the National Basic Research Program of China(973 Program)(2018YFA0901800) the National Natural Science Foundation of China(22138006,21736002,and 22078020) 

主　　题：constructing designing mining 

摘      要：Microbial cell factories (MCFs) are typical and widely used platforms in biomanufacturing for designing and constructingsynthesis pathways of target compounds in microorganisms. In MCFs, transporter engineering is especially significant forimproving the biomanufacturing efficiency and capacity through enhancing substrate absorption, promoting intracellular masstransfer of intermediate metabolites, and improving transmembrane export of target products. This review discusses thecurrent methods and strategies of mining and characterizing suitable transporters and presents the cases of transporterengineering in the production of various chemicals in MCFs.