Fermentation of rice straw raw material to bioethanol using consortium enzyme

作     者：Lailatul Fithri Luddy Febriyanto One Asmarani Rahmat Eko Sanjaya Ardiana Ilham Nurrohman Mirni Lamid Abdul Munir Abd Murad Ni Nyoman Tri Puspaningsih 

作者机构：1Proteomic Laboratory University-CoE-Research Center for Bio-Molecule Engineering Universitas Airlangga Surabaya 60115 Indonesia 2Departement of Chemistry Faculty of Science and Technology Universitas Airlangga Surabaya 60115 Indonesia 3Doctoral Program of Mathematics and Natural Sciences Faculty of Science and Technology Universitas Airlangga Surabaya 60115 Indonesia 4Animal Husbandry Faculty of Veterinery Medicine Universitas Airlangga Surabaya 60115 East Java Indonesia 5School of Biosciences and Biotechnology Faculty of Science and Technology Universiti Kebangsaan Malaysia Bangi 43600 Selangor Malaysia 

出 版 物：《AIP Conference Proceedings》 

年 卷 期：2023年第2679卷第1期

学科分类：07[理学] 0702[理学-物理学] 

摘      要：Rice straw is one of the abundant lignocellulosic agricultural by-product in Indonesia. Rice straw has several characteristics that make it a potential raw material for bioenergy production such as ethanol. It contain high cellulose and hemicelluloses that can be hydrolyzed into fermentable sugars. This study aimed to hydrolyze rice straw cellulose and hemicellulose using xylanase, cellulose, and xylose isomerase enzymes. 2% H2SO4 was used in pretreatment before the rice straw hydrolyzed with consortium enzymes. Concentrations of rice straw substrate was made to 5%, 10%, 15%, and 20% (w/v) and also made 1:1, 1:2, and 1:3 volume ratio between substrate and enzymes. The results showed that the highest rate of bioethanol obtained from 10% (w/v) rice straw substrate and enzyme activity in consortium was 0.279 U/mL (xylanase), 0.329 U/mL (cellulase), and 0.024 U/mL (xylose isomerase). The hydrolyzate was fermented by Saccharomyces cerevisiae BJ1824 and produce 8.81x10−2 %(v/v) ethanol measured by Gas Chromatography (GC).