Functional analysis of flavanone 3-hydroxylase(F3H)from Dendrobium officinale,which confers abiotic stress tolerance

作     者：Can Si Wei Dong Jaime A.Teixeira da Silva Chunmei He Zhenming Yu Mingze Zhang Lei Huang Conghui Zhao Danqi Zeng Chuanmao Li Shengxiang Qiu Jun Duan Can Si;Wei Dong;Jaime A.Teixeira da Silva;Chunmei He;Zhenming Yu;Mingze Zhang;Lei Huang;Conghui Zhao;Danqi Zeng;Chuanmao Li;Shengxiang Qiu;Jun Duan

作者机构：Key laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement&Guangdong ProvincialKey laboratory of Applied BotanySouth China Botanical GardenChinese Academy of SciencesGuangzhouGuangdong 510650China University of Chinese Academy of SciencesBeijing 100049China College of Life Science and TechnologyXi An Jiao Tong UniversityXi’anShaanxi 710049China Independent ResearcherMiki-choKagawa-kenJapan Guangzhou Keneng Cosmetic Scientific Research Co.LtdGuanghzouGuangdong 510650China 

出 版 物：《Horticultural Plant Journal》 (园艺学报（英文版）)

年 卷 期：2023年第9卷第2期

页      面：356-364页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 10[医学] 

基　　金：supported by the National Natural Science Foundation of China(Grant No.31871547) 

主　　题：Dendrobium officinale Flavanone 3-hydroxylase Dihydrokaempferol Salt stress Cold stress 

摘      要：Flavonoids are important bioactive components in Dendrobium officinale,a medicinal *** are involved in many biological activities,including protecting plants against biotic and abiotic *** on the key genes related to flavonoid biosynthesis in *** is *** this study,one of the key flavonoid biosynthesis genes,flavanone 3-hydroxylase(F3H),was characterized from *** open reading frame of DoF3H was 1134 bp long and it encoded a 377-amino acid *** DoF3H protein showed considerably high homology with F3H proteins from other plant species and shared a common evolutionary ancestor with other ***3H transcripts were detected in different organs of adult plants and mainly accumulated in flowers,followed by roots,stems and leaves,a pattern that was similar to the content of *** DoF3H protein,which was localized in the cytosol,could convert naringenin to *** mRNA levels of DoF3H were significantly induced by salt and cold ***,the heterologous expression of DoF3H in Escherichia coli conferred it higher tolerance to salt and cold *** results provide insight into the molecular function of DoF3H in the biosynthesis of flavonoids,and provide a new application for improvement of abiotic tolerance in ***.