De novo transcriptome assembly and metabolomic analysis of three tissue types in Cinnamomum cassia

作     者：Hongyang Gao Huiju Zhang Yuqing Hu Danyun Xu Sikai Zheng Shuting Su Quan Yang Hongyang Gao;Huiju Zhang;Yuqing Hu;Danyun Xu;Sikai Zheng;Shuting Su;Quan Yang

作者机构：School of Traditional Chinese MedicineGuangdong Pharmaceutical UniversityGuangzhou 510006China Key Laboratory of Production&Development of Cantonese Medicinal MaterialsState Administration of Traditional Chinese MedicineGuangzhou 510006China Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal MaterialsGuangzhou 510006China Comprehensive Experimental Station of GuangzhouChinese Material MedicaChina Agriculture Research System(CARS-21-16)Guangzhou 510006China 

出 版 物：《Chinese Herbal Medicines》 (中草药（英文版）)

年 卷 期：2023年第15卷第2期

页      面：310-316页

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：The Guangdong Basic and Applied Basic Research Foundation(No.2019A1515111043) Basic Research Project of Luoding Cinnamon Industry Development(No.2018-082) Youth Innovative Talents Project by Educational Department of Guangdong Province(No.2019KQNCX057) Special Fund for Science and Technology Innovation Cultivation of Guangdong University Students(special fund for“climbing plan”)(No.51348227) the Scientific Research Project of the Traditional Chinese Medicine Bureau of Guangdong Province(No.20202104)supported this study 

主　　题：Cinnamomum cassia Presl metabolomic phenylpropanoid biosynthesis transcriptome 

摘      要：Objective:The barks,leaves,and branches of Cinnamomum cassia have been historically used as a traditional Chinese medicine,spice,and food preservative,in which phenylpropanoids are responsible *** phenylpropanoid biosynthesis pathways are not clear in *** elucidated the pathways by descriptive analyses of differentially expressed genes related to phenylpropanoid biosynthesis as well as to identify various phenylpropanoid ***:Chemical analysis,metabolome sequencing,and transcriptome sequencing were performed to investigate the molecular mechanisms underlying the difference of active components content in the barks,branches and leaves of ***:Metabolomic analysis revealed that small amounts of flavonoids,coumarine,and cinnamaldehyde accumulated in both leaves and *** analysis showed that genes associated with phenylpropanoid and flavonoid biosynthesis were downregulated in the leaves and branches relative to the *** observed differences in essential oil content among the three tissues may be attributable to the differential expression of genes involved in the phenylpropanoid and flavonoid metabolic ***:This study identified the key genes in the phenylpropanoid pathway controling the flavonoid,coumarine,and cinnamaldehyde contents in the barks,branches and leaves by comparing the transcriptome and *** findings may be valuable in assessing phenylpropanoid and flavonoid metabolites and identifying specific candidate genes that are related to the synthesis of phenylpropanoids and flavonoids in ***.