A Novel Poly(3-hexylthiophene) Engineered Interface for Electrochemical Monitoring of Ascorbic Acid During the Occurrence of Glutamate-Induced Brain Cytotoxic Edemas

作     者：Zexuan Meng Yuchan Zhang Lu Yang Shuang Zhao Qiang Zhou Jiajia Chen Jiuxi Sui Jian Wang Lizhong Guo Luyue Chang Jialing He Guixue Wang Guangchao Zang Zexuan Meng;Yuchan Zhang;Lu Yang;Shuang Zhao;Qiang Zhou;Jiajia Chen;Jiuxi Sui;Jian Wang;Lizhong Guo;Luyue Chang;Jialing He;Guixue Wang;Guangchao Zang

作者机构：Institute of Life Scienceand Laboratory of Tissue and Cell BiologyLab Teaching and Management CenterChongqing Medical UniversityChongqing 400016China Key Laboratory for Biorheological Science and Technology of Ministry of EducationState and Local Joint Engineering Laboratory for Vascular ImplantsBioengineering College of Chongqing UniversityChongqing 400030China Jinfeng LaboratoryChongqing 401329China Department of PathophysiologyChongqing Medical UniversityChongqingChina 

出 版 物：《Research》 (研究（英文）)

年 卷 期：2024年第2023卷第1期

页      面：301-313页

核心收录：

学科分类：082803[工学-农业生物环境与能源工程] 08[工学] 0828[工学-农业工程] 0804[工学-仪器科学与技术] 

基　　金：the Natural Science Foundation of Chongqing(cstc2020jcyj-msxmX0330) the JinFeng Laboratory Foundation,Chongqing,China(jfkyjf202203001) the Project of Tutorial System of Medical Undergraduate in Lab Teaching and Management Center in Chongqing Medical University(LTMCMTS202109 and LTMCMTS202106) the Project of Scientific Research and Innovative Experiment for College Student in Chongqing Medical University(SRIEP202011,SRIEP202047,and SPIEP202167) the National Project of University Students Innovation and Entrepreneurship Training Program(S202010631016) Chongqing Municipal Education Commission Foundation(CYS22333) 

主　　题：release microelectrode Engine 

摘      要：Although neuroelectrochemical sensing technology offers unique benefits for neuroscience research,its application is limited by substantial interference in complex brain environments while ensuring biosafety *** this study,we introduced poly(3-hexylthiophene)(P3HT)and nitrogen-doped multiwalled carbon nanotubes(N-MWCNTs)to construct a composite membrane-modified carbon fiber microelectrode(CFME/P3HT-N-MWCNTs)for ascorbic acid(AA)*** microelectrode presented good linearity,selectivity,stability,antifouling,and biocompatibility and exhibited great performance for application in neuroelectrochemical ***,we applied CFME/P3HT-N-MWCNTs to monitor AA release from in vitro nerve cells,ex vivo brain slices,and in vivo living rat brains and determined that glutamate can induce cell edema and AA *** also found that glutamate activated the N-methyl-d-aspartic acid receptor,which enhanced Na^(+) and Cl^(−) inflow to induce osmotic stress,resulting in cytotoxic edema and ultimately AA *** study is the first to observe the process of glutamate-induced brain cytotoxic edema with AA release and to reveal the *** work can benefit the application of P3HT in in vivo implant microelectrode construction to monitor neurochemicals,understand the molecular basis of nervous system diseases,and discover certain biomarkers of brain diseases.