Anti-F-Actin Antibody Positivity on Indirect Immunofluorescence Assay Following Chinese and Alternative Medicine Therapy: A Case Report

作     者：Mala Mahto Neha Rai Soma Dey Ramesh Kumar 

作者机构：Department of Biochemistry All India Institute of Medical Sciences (AIIMS) Patna Patna Bihar India Department of Gastroenterology All India Institute of Medical Sciences (AIIMS) Patna Patna Bihar India 

出 版 物：《Journal of Laboratory Physicians》 

年 卷 期：2022年第15卷第2期

页      面：311-315页

主　　题：anti F actin antibody indirect immunofluorescence drug induced autoimmune liver disease giloy 

摘      要：Immunofluorescence on human epithelial type 2 cells is the standard screening assay for the detection of antinuclear antibodies (ANA). Cytoplasmic speckled patterns are a common finding. However, the less commonly reported ones include the cytoplasmic fibrillar patterns on indirect immunofluorescence technique (IIFT). The cytoplasmic fibrillar patterns include the cytoplasmic linear (AC-15), cytoplasmic filamentous (AC-16), and cytoplasmic segmental (AC-17). We report a case of cytoplasmic linear (F-actin) detected through IIFT during ANA screening in a 77-year-old man and later reconfirmed on liver mosaic biochip through IIFT on vascular smooth muscle substrate (VSM-47) without features suggestive of anti-smooth muscle antibody involvement post-complementary and alternative medicine therapy initiation.