PGAM5 deacetylation mediated by SIRT2 facilitates lipid metabolism and liver cancer proliferation

作     者：Gongyu Fu Shi-Ting Li Zetan Jiang Qiankun Mao Nanchi Xiong Xiang Li Yijie Hao Huafeng Zhang Gongyu Fu;Shi-Ting Li;Zetan Jiang;Qiankun Mao;Nanchi Xiong;Xiang Li;Yijie Hao;Huafeng Zhang

作者机构：Anhui Key Laboratory of Hepatopancreatobiliary SurgeryDepartment of General SurgeryAnhui Provincial Hospitalthe First Affiliated Hospital of USTCDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefei 230027China Hefei National Laboratory for Physical Sciences at Microscalethe Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefei 230027China Guangdong Cardiovascular InstituteGuangdong Provincial People's HospitalGuangdong Academy of Medical SciencesGuangzhou 510080China Hefei National Laboratory for Physical Sciences at Microscalethe Chinese Academy of Sciences Key Laboratory of Innate Immunity and Chronic DiseaseSchool of Basic Medical SciencesDivision of Life Science and MedicineUniversity of Science and Technology of ChinaHefei 230027China 

出 版 物：《生物化学与生物物理学报（英文版）》 (Acta Biochimica et Biophysica Sinica)

年 卷 期：2023年第55卷第9期

页      面：1370-1379页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：This work was supported by the grants from the National Natural Science Foundation of China (Nos. 81930083  82192893  82103363  and 81821001)  the Chinese Academy of Sciences (No. XDB39000000)  the National Key R&D Program of China (No. 2022YFA1304504)  the Institute of Health and Medicine  Hefei Comprehensive National Science Center (No. DJK-LX-2022001)  the Fundamental Research Funds for the Central Universities (No. YD2070002008)  and the China Postdoctoral Science Foundation (No. 2021M700898) 

主　　题：metabolic reprograming PGAM5 protein acetylation SIRT2 ME1 liver cancer 

摘      要：Tumor metabolic reprogramming and epigenetic modification work together to promote tumorigenesis and *** lysine acetylation,which affects a variety of biological functions of proteins,plays an important role under physiological and pathological ***,through immunoprecipitation and mass spectrum data,we show that phosphoglycerate mutase 5(PGAM5)deacetylation enhances malic enzyme 1(ME1)metabolic enzyme activity to promote lipid synthesis and proliferation of liver cancer ***,we demonstrate that the deacetylase SIRT2 mediates PGAM5 deacetylation to activate ME1 activity,leading to ME1 depho-sphorylation,subsequent lipid accumulation and the proliferation of liver cancer *** together,our study establishes an important role for the SIRT2-PGAM5-ME1 axis in the proliferation of liver cancer cells,suggesting a potential innovative cancer therapy.