JNK/MAPK pathway regulation by BEX2 gene silencing in alcoholic hepatitis mice: Effects on oxidative stress

作     者：Zhou, Shuai Zhong, Hai Wang, Yong Wang, Xiaoguang Pan, Hongtao Liu, Xiaolin Hu, Lingyu 

作者机构：Anhui Med Univ Hosp 2 Dept Gen Surg Hefei Anhui Peoples R China Jiaxing Univ Affiliated Hosp 2 Dept Surg Jiaxing Peoples R China Jiaxing Univ Affiliated Hosp 2 Dept Surg 1518 North Huancheng Rd Jiaxing 314000 Zhejiang Peoples R China 

出 版 物：《ALCOHOL-CLINICAL AND EXPERIMENTAL RESEARCH》 (酒精中毒；临床与实验研究)

年 卷 期：2023年第47卷第10期

页      面：1869-1882页

核心收录：

学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：The authors have nothing to report 

主　　题：alcoholic hepatitis brain expressed X-linked gene 2 (BEX2) gene silencing JNK/MAPK pathway liver tissue microarray dataset oxidative stress 

摘      要：Background: Alcoholic hepatitis (AH) is a severe alcoholic-related liver disease that is a leading cause of morbidity and mortality, for which effective treatments are lacking. Brain-expressed X-linked gene 2 (BEX2) has been implicated in various diseases, but its association with AH has received limited attention. Thus, this study investigated BEX2 s impact on the progression of AH by affecting the c-Jun NH2-terminal kinase/mitogen-activated protein kinase (JNK/MAPK) ***: Microarray dataset GSE28619 from the Gene Expression Omnibus database was used to identify differentially expressed genes in AH. Immunohistochemistry, terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL), Western blot analysis, and flow cytometry were used to measure various factors in the liver tissue of AH ***: BEX2 expression was significantly upregulated in the model. BEX2 gene silencing increased the levels of glutathione peroxidase and superoxide dismutase while decreasing malondialdehyde content;phosphorylation of JNK, c-JUN, and p38MAPK;apoptosis rate;and the extent of JNK/MAPK pathway ***: These findings provide valuable insights into the mechanisms underlying AH development and highlight the potential role of BEX2 gene expression as a promising therapeutic target for AH.