Rapid accuracy determining DNA purity and concentration in heavy oils by spectrophotometry methods

作     者：YunYang Wan HongMei Mu Na Luo JianPing Yang Yan Tian Ning Hong HaiLiang Dong 

作者机构：National Key Laboratory of Petroleum Resources and EngineeringResearch Centre for Geomicrobial Resources and ApplicationBeijing Key Laboratory of Petroleum Pollution and ControlUnconventional Petroleum Research InstituteChina University of PetroleumBeijing102249China Drilling and Production Engineering Technology Department of PetroChina Liaohe Oilfield CompanyPanjin124010LiaoningChina Center for Geomicrobiology and Biogeochemistry ResearchState Key Laboratory of Biology and Environmental GeologyChina University of GeosciencesBeijing100083China 

出 版 物：《Petroleum Science》 (石油科学（英文版）)

年 卷 期：2023年第20卷第6期

页      面：3394-3399页

核心收录：

学科分类：081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 

基　　金：supported by grants from the PetroChina-CUP Major Strategic Cooperation Projects(ZLZX2020010805,ZLZX2020020405) National Natural Science Foundation of China(41373086) National Science and Technology Major Project(No.2016ZX05050011,2016ZX05040002) Beijing Nova Program and Leading Talent Culturing Cooperative Projects(No.Z161100004916033) Beijing Higher Education Young Elite Teacher Project(No.YETP0670) Outstanding Young Excellent Teachers Foundation of China University of Petroleum(Beijing)(KYJJ2012-01-10) 

主　　题：Heavy oil DNA concentration DNA purity Spectrophotometry qPCR 

摘      要：DNA analysis is the core of biotechnology applied in petroleum resources and engineering. Traditionally accurate determination of DNA purity and concentration by spectrometer is the first and critical step for downstream molecular biology research. In this study, three different spectrophotometry methods, BPM, NDTT and NPMTTZ were compared for their performance in determining DNA concentration and purity in 32 oil samples, and molecule methods like quantitative real-time PCR (qPCR) and high-throughput sequence were also performed to help assess the accuracy of the three methods in determining DNA concentration and purity. For ordinary heavy oil (OHO), extra heavy oil (EHO) and super heavy oil (SHO), the characteristics of high viscosity (η), density (ρ) and resin plus asphaltene content will affect the DNA extraction and UV determination. The DNA concentration was decreased as density increased: OHO (11.46 ± 18.34 ng/μL), EHO (6.68 ± 9.67 ng/μL) and SHO (6.20 ± 7.83 ng/μL), and the DNA purity was on the reverse: OHO (1.31 ± 0.27), EHO (1.54 ± 0.20), and SHO (1.83 ± 0.32). The results suggest that spectrophotometry such as BPM and NPMTTZ are qualitatively favorite methods as the quick non-consumable methods in determining DNA concentration and purity of medium oil and heavy oil.