KINETIC STUDIES OF (CA-2+) OR (MG-2-) ATPASE ACTIVITY OF LYMPHOCYTE PLASMA

作     者：PAU, B DORNAND, J MANI, JC 

作者机构：ECOLE NATL SUPER CHIM EQUIPE RECH CNRS 62 F-34075 MONTPELLIER FRANCE 

出 版 物：《BIOCHIMIE》 (Biochimie)

年 卷 期：1976年第58卷第5期

页      面：593-599页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

摘      要：The kinetic study of the (Ca2+) ATPase activity of lymphocyte plasma membranes allowed some properties of this enzyme to be evidenced. The Ca2+-activated hydrolysis of ATP is independent of a non-specific alkaline phosphatase. The substrate of the ATPase activity is the chelate Ca2+-ATP. Mg2+ may substitute for Ca2+ both as chelating ion and as activating ion. There is apparently only 1 ATPase, activated either by Ca2+, or by Mg2+ with less efficiency. Both chelates have the same Km;pH values for maximum activity and transition temperatures are identical. The effects of free ions are also the same, activation at low concentration and inhibition at high concentration.