Contrast-enhanced fluorescence microscope by LED integrated excitation cubes

作     者：Yuanhua Liu Xiang Zhang Fei Su Zhiyong Guo Dayong Jin 

作者机构：UTS-SUSTech Joint Research Centre for Biomedical Materials and DevicesDepartment of Biomedical EngineeringSouthern University of Science and TechnologyShenzhenGuangdong 518055China Institute for Biomedical Materials and Devices(IBMD)Faculty of ScienceUniversity of Technology SydneyNSW 2007Australia 

出 版 物：《Light(Advanced Manufacturing)》 (光（先进制造）（英文）)

年 卷 期：2023年第4卷第2期

页      面：18-27页

学科分类：08[工学] 0803[工学-光学工程] 

基　　金：the Shenzhen Science and Technology Program(KQTD20170810110913065 and 20200925174735005) 

主　　题：LED-integrated excitation cube Filter cube Time-gated imaging Fluorescence microscopy 

摘      要：Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical *** compensate for the short operation lifetime,integrated light sources,and low excitation efficiency of conventional light sources such as mercury,halogen,and xenon lamps,we designed an LED-integrated excitation cube(LEC)with a decentralized structure and high optical power *** a Fresnel lens,the light from the light-emitting diode(LED)was effectively focused within a 15 mm mounting distance to achieve high-efficiency *** can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence ***’optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps;therefore,high-quality fluorescence imaging with spectral coverage from UV to red can be *** replacing conventional fluorescence filter cubes,LEC can be easily installed on any commercial fluorescence microscope.A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of ***,high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.