Metabolites and metabolic pathway analysis of sulfadimidine in carp (Cyprinus carpio) based on UHPLC-Q-orbitrap HRMS

作     者：Wang, Shuwen Xing, Lihong Sun, Xiaojie Li, Zhaoxin Ding, Tao Wang, Jiyao Peng, Jixing Ma, Haijian Lin, Ting Yang, Qingli 

作者机构：Chinese Acad Fishery Sci Yellow Sea Fisheries Res Inst Key Lab Testing & Evaluat Aquat Prod Safety & Qual Minist Agr & Rural Affairs 106 Nanjing Rd Qingdao 266071 Peoples R China ANPEL Lab Technol Shanghai Peoples R China Qingdao Agr Univ Coll Food Sci & Engn Qingdao Peoples R China 

出 版 物：《ENVIRONMENTAL RESEARCH》 (环境研究)

年 卷 期：2024年第252卷第Part3期

页      面：118967页

核心收录：

学科分类：12[管理学] 1204[管理学-公共管理] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 120402[管理学-社会医学与卫生事业管理(可授管理学、医学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：National Key R & D Program "Blue Granary Science and Technology Innovation" Key Project "Detection and Quality Control Technology of Hazardous Substances in Aquatic Products" [2019YFD0901701] Shandong Natural Science Foundation Project [ZR2023MD109] Basic Scientific Research Business Fund Project of Chinese Academy of Fishery Sciences [2023TD76] 

主　　题：Sulfadimidine Cyprinus carpio UHPLC-Q-exactive plus orbitrap-MS Metabolites Metabolic pathways 

摘      要：Sulfadimidine (SM2) is an N-substituted derivative of p-aminobenzenesulfonyl structure. This study aimed to analyze the metabolism of SM2 in carp (Cyprinus carpio). The carps were fed with SM2 at a dose of 200 mg/(kg bw) and then killed. The blood, muscle, liver, kidney, gill, other guts, and carp aquaculture water samples were collected. The UHPLC-Q-Exactive Plus Orbitrap-MS was adopted for determining the metabolites of SM2 in the aforementioned samples. Twelve metabolites, which were divided into metabolites in vivo and metabolites in vitro, were identified using Compound Discoverer software. The metabolic pathways in vivo of SM2 in carp included acetylation, hydroxylation, glucoside conjugation, glycine conjugation, carboxylation, glucuronide conjugation, reduction, and methylation. The metabolic pathways in vitro included oxidation and acetylation. This study clarified the metabolites and metabolic pathways of SM2 in carp and provided a reference for further pharmacodynamic evaluation and use in aquaculture.