The Mechanisms of Yeast Fermentation Extracellular Metabolites In Stimulating Microbial Degradation of Trichloroethylene: Physiological Characteristics and Omics Analysis

作     者：Yuan, Meng Chen, Guotao Xiao, Yibo Qu, Yujiao Ren, Yuan 

作者机构：School of Environment and Energy South China University of Technology Guangzhou510006 China Protoga Biotechnology Co. Ltd. Shenzhen518000 China Microalgae Biosynthesis R&D Center Research Institute of Tsinghua University in Shenzhen Shenzhen518057 China The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters Ministry of Education Guangzhou510006 China The Key Laboratory of Environmental Protection and Eco-Remediation of Guangdong Regular Higher Education Institutions Guangzhou510006 China 

出 版 物：《SSRN》 

年 卷 期：2024年

核心收录：

主　　题：Biodegradation 

摘      要：Trichloroethylene (TCE) is a common chlorinated organic pollutant in the environment. The biodegradation of TCE is limited by low microbial metabolic activity but can be enhanced through biostimulation strategies. This study explored the physiological effects and potential molecular mechanisms of yeast fermentation extracellular metabolites (YFEMs) on the degradation of TCE by Acinetobacter LT1. Results indicated that YFEMs stimulated the efficiency of LT1 strain by 50.28%. At the physiological level, YFEMs exhibited protective effects on cell morphology, reduced oxidative stress, lessened membrane damage, and enhanced energy production and conversion. Analysis of genomic and proteomic results revealed that YFEMs’ stimulation effect on LT1 primarily involved processes of detoxification, energy conversion, and transport. Furthermore, RT-qPCR results showed significant upregulation in the genes encoding YhhW protein which has quercetin 2,3-dioxygenase activity in both TCE stress and YFEMs stimulation groups, which were 1.72 and 3.22 times the control group, respectively. Molecular docking results discovered hydrophobic interactions between TCE and YhhW, leading to a conformational change into a more active form of the protein, thereby enhancing the enzyme s activity. Therefore, YhhW is hypothesized to be a primary enzyme system in the TCE degradation process, with the addition of YFEMs further stimulating its activity. These results reveal the mechanism by which YFEMs induce strain LT1 to enhance TCE degradation and propose the potential degradative capacity of YhhW towards TCE, providing scientific insights for the biodegradation of TCE. © 2024, The Authors. All rights reserved.