Integrated proteomic,transcriptomic,and metabolomic profiling reveals that the gibberellin-abscisic acid hub runs flower development in the Chinese orchid Cymbidium sinense

作     者：Sagheer Ahmad Chuqiao Lu Jie Gao Yonglu Wei Qi Xie Jianpeng Jin Genfa Zhu Fengxi Yang Sagheer Ahmad;Chuqiao Lu;Jie Gao;Yonglu Wei;Qi Xie;Jianpeng Jin;Genfa Zhu;Fengxi Yang

作者机构：Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and UtilizationEnvironmental Horticulture Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China Guangdong Key Laboratory of Ornamental Plant Germplasm Innovation and UtilizationEnvironmental Horticulture Research InstituteGuangdong Academy of Agricultural SciencesGuangzhou 510640China Guangdong Laboratory for Lingnan Modern AgricultureGuangzhou 510640China 

出 版 物：《园艺研究（英文）》 (Horticulture Research)

年 卷 期：2024年第11卷第5期

页      面：163-179页

核心收录：

基　　金：国家自然科学基金 the Guangzhou Science and Technology Project the Innovation Team of Modern Agriculture Industry Technology System in Guangdong Province the Guangdong Academy of Agricultural Sciences Discipline Team Construction Project 

摘      要：The seasonal flowering Chinese Cymbidium produce an axillary floral meristem and require a dormancy period during cold conditions for flower ***,the bud activation mechanism remains *** study evaluates the multi-omics across six stages of flower development,along with functional analysis of core genes to decipher the innate mechanism of floral bud initiation and outgrowth in the Chinese orchid Cymbidium *** and proteome analyses identified 10 modules with essential roles in floral bud dormancy and *** clusters in the early stages of flower development were mainly related to flowering time regulation and meristem determination,while the late stages were correlated with hormone signaling *** metabolome identified 69 potential hormones in which gibberellin(GA)and abscisic acid(ABA)were the main regulatory hubs,and GA4 and GA53 exhibited a reciprocal *** GA application caused rapid elongation of flower buds and promoted the expression of flower development ***,exogenous ABA application extended the dormancy process and ABA inhibitors induced dormancy ***,CsAPETALA1(CsAP1)was identified as the potential target of ABA for floral bud *** of CsAP1 in Arabidopsis and its transient overexpression in *** protoplasts not only affected flowering time and floral organ morphogenesis in Arabidopsis but also orchestrated the expression of flowering and hormone regulatory *** presence of ABA response elements in the CsAP1 promoter,rapid downregulation of CsAP1 after exogenous ABA application,and the activation of the floral bud after ABA inhibitor treatment suggest that ABA can control bud outgrowth through CsAP1.