Dietary supplementation with Neolamarckia cadamba leaf extract improves broiler meat quality by enhancing antioxidant capacity and regulating metabolites

作     者：Cheng Wang Dandan Chen Shou Wu Wei Zhou Xiaoyang Chen Qing Zhang Li Wang Cheng Wang;Dandan Chen;Shou Wu;Wei Zhou;Xiaoyang Chen;Qing Zhang;Li Wang

作者机构：State Key Laboratory of Swine and Poultry Breeding IndustryKey Laboratory of Animal Nutrition and Feed Science in South ChinaMinistry of Agriculture and Rural AffairsGuangdong Provincial Key Laboratory of Animal Breeding and NutritionMaoming BranchGuangdong Laboratory for Lingnan Modern AgricultureInstitute of Animal ScienceGuangdong Academy of Agricultural SciencesGuangzhou 510640China College of Forestry and Landscape ArchitectureGuangdong Province Research Center of Woody Forage Engineering TechnologyGuangdong Research and Development Center of Modern Agriculture(Woody Forage)Industrial TechnologyGuangdong Key Laboratory for Innovative Development and Utilization of Forest Plant GermplasmState Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresourcesSouth China Agricultural UniversityGuangzhou 510642China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2024年第17卷第2期

页      面：358-372页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National Key R&D Program of China(Grant No.2022YFE0111000) the central government guides local funds for science and technology development–basic research projects with free exploration(2022ZY0152) the Science and Technology Program of Guangdong Academy of Agricultural Sciences(Grant No.202106TD) 

主　　题：Neolamarckia cadamba leaf extract Broiler Meat quality Antioxidant capacity Metabolomics 

摘      要：This study was to evaluate the effect of supplementing the diet of broilers with Neolamarckia cadamba leaf extract(NCLE)on meat quality by evaluating antioxidant parameters and the expression of genes in the p38 mitogen-activated protein kinase/nuclear factor-erythroid 2-related factor 2/antioxidant responsive element(p38 MAPK/Nrf2/ARE)signaling pathway,coupled with LC-MS-based metabolomic analysis.A total of 480 one-day-old male broilers were randomly allocated to four treatment groups-a control(CON)group,which was fed a basal diet,and three NCLE treatment groups,which were fed the basal diet sup-plemented with 100,200,or 400 mg/kg NCLE(N1,N2,and N3 groups,respectively)for 42 *** with the CON group,meat quality was improved in the N2 and N3 groups,as evidenced by the higher pH45min(P0.05)and lower shear force(P0.05)in breast muscle(BM)and lower drip loss at 48 h(P0.05)in leg muscle(LM).Moreover,BM antioxidant capacity was significantly enhanced in the N3 group,characterized by an increase in the total antioxidant capacity(T-AOC),the concentrations of glutathione peroxidase(GSH-Px)and catalase(CAT),and the relative mRNA expression of p38 MAPK,extracellular-signal regulated kinase 1/2(ERK1/2),c-Jun N-terminal kinase(JNK),Nrf2,CAT,and GSH-Px(P0.05).Similarly,LM in the N3 group displayed higher T-AOC,increased GSH-Px and CAT concentrations,reduced malonaldehyde contents(P0.05),and upregulation of the relative mRNA levels of JNK,Nrf2,heme oxygenase,CAT,and superoxide dismutase(SOD)(P0.05).Metabolomics analysis revealed that D-arabinono-1,4-lactone and lyso-PAF C-16-d4 were negatively correlated with shear force and cooking loss(P0.05)and displayed increased abundance in BM of the N3 group.L-Serine levels were upregulated while D-fructose 1,6-diphosphate contents were downregulated in the three NCLE ***,the differential metabolites in both BM and LM were involved in amino acid metabolism *** results indicated that NCLE supplementation im