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作者机构:Univ Groningen Groningen Biomol Sci & Biotechnol Inst GBB Microbial Physiol Nijenborgh 7 NL-9747 AG Groningen Netherlands Eurofins Expertise Ctr Complex Carbohydrates & Che POB 766 NL-8440 AT Heerenveen Netherlands Univ Groningen Univ Med Ctr Groningen Dept Lab Med Hanze pl 1EA30 NL-9713 GZ Groningen Netherlands Royal FrieslandCampina Stations pl 4 NL-3818 LE Amersfoort Netherlands CarbExplore Res BV Zernikelaan 8 NL-9747 AA Groningen Netherlands Univ Appl Sci Van Hall Larenstein POB 1528Agora 1 NL-8901 BV Leeuwarden Netherlands
出 版 物:《APPLIED MICROBIOLOGY AND BIOTECHNOLOGY》 (应用微生物学与生物技术)
年 卷 期:2024年第108卷第1期
页 面:349-349页
核心收录:
学科分类:0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 07[理学] 0836[工学-生物工程] 071005[理学-微生物学] 10[医学]
主 题:Galacto-oligosaccharides Gluco-oligosaccharides Thermostable Glucosidase Galactosidase
摘 要:Galacto-oligosaccharides (GOS) are prebiotic compounds that are mainly used in infant formula to mimic bifidogenic effects of mother s milk. They are synthesized by beta-galactosidase enzymes in a trans-glycosylation reaction with lactose. Many beta-galactosidase enzymes from different sources have been studied, resulting in varying GOS product compositions and yields. The in vivo role of these enzymes is in lactose hydrolysis. Therefore, the best GOS yields were achieved at high lactose concentrations up to 60%wt, which require a relatively high temperature to dissolve. Some thermostable beta-glucosidase enzymes from thermophilic bacteria are also capable of using lactose or para nitrophenyl-galactose as a substrate. Here, we describe the use of the beta-glucosidase BglA from Thermotoga maritima for synthesis of oligosaccharides derived from lactose and cellobiose and their detailed structural characterization. Also, the BglA enzyme kinetics and yields were determined, showing highest productivity at higher lactose and cellobiose concentrations. The BglA trans-glycosylation/hydrolysis ratio was higher with 57%wt lactose than with a nearly saturated cellobiose (20%wt) solution. The yield of GOS was very high, reaching 72.1%wt GOS from lactose. Structural elucidation of the products showed mainly beta(1 - 3) and beta(1 - 6) elongating activity, but also some beta(1 - 4) elongation was observed. The beta-glucosidase BglA from T. maritima was shown to be a very versatile enzyme, producing high yields of oligosaccharides, particularly GOS from lactose.