A homogeneous binary matrix assisted laser desorption/ionization time-of-flight mass spectrometry assay for determination of artificial sweeteners in beverages

作     者：Wang, Mengzhen Ling, Ling Wang, Shuyi Ding, Chuan-Fan 

作者机构：Ningbo Univ Inst Mass Spectrometry Sch Mat Sci & Chem Engn Key Lab Adv Mass Spectrometry & Mol Anal Zhejiang Ningbo 315211 Zhejiang Peoples R China 

出 版 物：《FOOD CHEMISTRY》 (食品化学)

年 卷 期：2024年第460卷第Part2期

页      面：140597页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 08[工学] 0703[理学-化学] 

基　　金：National Key Research and Develop-ment Program of China [2023YFF0721004] National Natural Science Foundation of China Zhejiang Provincial Natural Science Foundation of China [LQ23B050005] 

主　　题：Artificial sweeteners Aspartame Nanodiamonds Binary matrix MALDI-TOF MS Beverage 

摘      要：Artificial sweeteners have been widely used as additives in various beverages. Due to the safety risks associated with artificial sweeteners, it is essential to develop a simple, rapid, and high-throughput method for the analysis of artificial sweeteners. Here, we report a homogeneous binary matrix assisted laser desorption/ionization time- of-flight mass spectrometry (MALDI-TOF MS) assay for the simultaneous analysis of sweeteners including aspartame (ASP), neotame (NEO), and advantame (ADV) with a simple dilution step. The combination of nanodiamonds with 2,5-dihydroxybenzoic acid effectively improved the signal response of sweeteners, decreased the background noise, and improved the spot-to-spot repeatability. After the optimization, the method exhibits low limits of detection (ASP: 20 n NEO: 10 n ADV: 5 n ), ), good linearity (r r 0.995), satisfactory accuracy (96.2-103.0%), and lower RSDs (1.5-5.8%). Finally, the target sweeteners in 17 soft beverages were successfully determined with this method, showing the potential for the routine analysis of artificial sweeteners.