Fluorescence Nanosensor Based on Modified Sustainable Silica for Highly Sensitive Detection of Sars-Cov-2 Igg Antibody

作     者：Apriyani, Firda Sari, Shaimah Rinda Petrus, Himawan Tri Bayu Murti Angelina, Marissa Manurung, Robeth Victoria Septiani, Ni Luh Wulan Yuliarto, Brian Jenie, S.N. Aisyiyah 

作者机构：Master Program of Nanotechnology Graduate School Institut Teknologi Bandung Ganesha 10 West Java Bandung40132 Indonesia Advanced Functional Material Research Group Faculty of Industrial Technology Institut Teknologi Bandung Jl. Ganesha No. 10 West Java Bandung41032 Indonesia  Kawasan Puspiptek Building 452 Serpong Banten South Tangerang15314 Indonesia Department of Chemical Engineering Faculty of Engineering Universitas Gadjah Mada Jalan Grafika No 2 Yogyakarta55281 Indonesia  West Java Bogor16911 Indonesia BRIN and ITB Collaboration Research Center for Biosensor and Biodevices Jl. Ganesa 10 West Java Bandung40132 Indonesia  Komplek LIPI Building. 20 Jl. Cisitu Lama Dago West Java Bandung40135 Indonesia  Kawasan Puspitek South Tangerang15314 Indonesia 

出 版 物：《SSRN》 

年 卷 期：2024年

核心收录：

主　　题：Coronavirus 

摘      要：COVID-19 continues to pose significant global health challenges, leading to continuous developments in detection, vaccination, and treatment strategies that require an accurate and rapid detection of severe acute respiratory syndrome coronavirus 2 immunoglobulin G (SARS-CoV-2 IgG) antibodies. Since its emergence in 2020, SARS-CoV-2 has undergone multiple mutations, leading to the development of new variants that necessitate updated vaccines and diagnostic methodologies. This study presents an innovative fluorescence nanosensor utilizing modified sustainable silica for the ultra-sensitive detection of SARS-CoV-2 IgG antibodies. The sensor employs fluorescent dye-doped silica nanoparticles (FSNP) synthesized via the sol-gel method and functionalized with rhodamine B as a fluorescence dye. Fourier-transform infrared (FTIR) analysis confirmed the successful immobilization of anti-IgG on the FSNP surface, as evidenced by characteristic amide I and II peaks at 1641 cm-1 and 1530 cm-1, respectively. Detection of SARS-CoV-2 IgG antibodies was achieved through enhanced fluorescence intensity of FSNP-anti-IgG at 582 nm. Optimal detection conditions were established with a 15-minute incubation period, demonstrating a linear detection range from 10-2 to 10-8 µg/mL and a limit of detection (LOD) of 5.3 fg/mL. This research highlights the potential of modified sustainable silica-based fluorescence nanosensors for advancing sensitive and rapid COVID-19 diagnostics. © 2024, The Authors. All rights reserved.