Modulating the Angiogenic and Immunomodulatory Capacities of Stem Cell-Derived Extracellular Vesicles in Culture Through Mechanical Microenvironment Alteration

作     者：Kang, Wu Young Jung, Sunyoung Jeong, Hyundoo Woo, Hyun-Myung Bae, Hojae Cha, Jae Min 

作者机构：Department of Biomedical & Robotics Engineering College of Engineering Incheon National University Incheon22012 Korea Republic of 3D Stem Cell Bioengineering Laboratory Research Institute for Engineering and Technology Incheon National University Incheon22012 Korea Republic of Department of Stem Cell and Regenerative Biotechnology KU Convergence Science and Technology Institute Konkuk University Seoul05029 Korea Republic of Institute of Advanced Regenerative Science Konkuk University Seoul05029 Korea Republic of 

出 版 物：《SSRN》 

年 卷 期：2024年

核心收录：

主　　题：Stem cells 

摘      要：Stem cell-derived extracellular vesicles (EVs) have attracted attention as a promising therapy which could address the limitations of current stem cell treatments. Nevertheless, the inarticulate and inconsistent therapeutic contents of EVs obtained by traditional stem cell culture hinder their clinical applications. This study explores the regulation of the therapeutic efficacy of EVs derived from mesenchymal stem cells (MSCs) through 3D hydrogel culture with controlled mechanical microenvironments. Our results reveal that different physical interactions between MSCs and culture matrices lead to distinct MSC gene expression patterns while preserving the MSCs’ own characteristics as stem cells. In addition, EVs collected from the MSCs showed differentiated angiogenic and immunomodulatory efficacies depending on the mechanical properties of the culture matrices, which was further supported by our comprehensive therapeutic cargo analyses of the cytokines and micro-RNAs in the EVs. As a non-invasive culture approach to stem cells, circumventing genetic modifications or additional biochemical supplementations, our methodology presents a novel avenue for the customized production of EV medicine, potentially advancing clinical applications in regenerative medicine. © 2024, The Authors. All rights reserved.