Enhanced multiscale human brain imaging by semi-supervised digital staining and serial sectioning optical coherence tomography

作     者：Shiyi Cheng Shuaibin Chang Yunzhe Li Anna Novoseltseva Sunni Lin Yicun Wu Jiahui Zhu Ann C.McKee Douglas L.Rosene Hui Wang Irving J.Bigio David A.Boas Lei Tian Shiyi Cheng;Shuaibin Chang;Yunzhe Li;Anna Novoseltseva;Sunni Lin;Yicun Wu;Jiahui Zhu;Ann C.McKee;Douglas L.Rosene;Hui Wang;Irving J.Bigio;David A.Boas;Lei Tian

作者机构：Department of Electrical and Computer EngineeringBoston UniversityBostonMA 02215USA Department of Electrical Engineering and Computer SciencesUniversity of CaliforniaBerkeleyCA 94720USA Department of Biomedical EngineeringBoston UniversityBostonMA 02215USA Department of Electrical and Computer EngineeringBoston UniversityBostonMA 02215USA Department of Biomedical EngineeringBoston UniversityBostonMA 02215USA Department of Computer ScienceBoston UniversityBostonMA 02215USA Boston University Alzheimer's Disease Research Center and CTE CenterBoston University School of MedicineBostonMA 02118USA Department of NeurologyBoston University School of MedicineBostonMA 02118USA VA Boston Healthcare SystemU.S.Department of Veteran AffairsBostonMA 02130USA Department of Pathology and Laboratory MedicineBoston University School of MedicineBostonMA 02118USA Department of Anatomy & NeurobiologyBoston University School of MedicineBostonMAUSA Department of RadiologyAthinoula A.Martinos Center for Biomedical ImagingMassachusetts General HospitalBostonMA 02129USA Department of Electrical and Computer EngineeringBoston UniversityBostonMA 02215USA Department of Biomedical EngineeringBoston UniversityBostonMA 02215USA Neurophotonics CenterBoston UniversityBostonMA 02215USA 

出 版 物：《光：科学与应用（英文版）》 (Light：Science & Applications)

年 卷 期：2025年第14卷第2期

页      面：490-508页

核心收录：

学科分类：070207[理学-光学] 07[理学] 08[工学] 0803[工学-光学工程] 0702[理学-物理学] 

基　　金：National Institutes of Health, NIH, (R01 EY032163, R01 NS125307, R01 NS128843, P30 AG072978, RF1 AG062831, U19 AG068753, U54 NS115266, R01 AG075727) National Institutes of Health, NIH Boston University, BU, (R01 NS128843, R00 EB023993, U01 MH117023) Boston University, BU 

主　　题：tomography serial imaging digital human brain coherence enhanced multiscale optical 

摘      要：A major challenge in neuroscience is visualizing the structure of the human brain at different *** histology reveals micro-and meso-scale brain features but suffers from staining variability,tissue damage,and distortion,which impedes accurate 3D *** emerging label-free serial sectioning optical coherence tomography(S-OCT)technique offers uniform 3D imaging capability across samples but has poor histological interpretability despite its sensitivity to cortical ***,we present a novel 3D imaging framework that combines S-OCT with a deep-learning digital staining(DS)*** enhanced imaging modality integrates high-throughput 3D imaging,low sample variability and high interpretability,making it suitable for 3D histology *** develop a novel semi-supervised learning technique to facilitate DS model training on weakly paired images for translating S-OCT to Gallyas silver *** demonstrate DS on various human cerebral cortex samples,achieving consistent staining quality and enhancing contrast across cortical layer ***,we show that DS preserves geometry in 3D on cubic-centimeter tissue blocks,allowing for visualization of meso-scale vessel networks in the white *** believe that our technique has the potential for high-throughput,multiscale imaging of brain tissues and may facilitate studies of brain structures.