Fibrotic and emphysematous murine lung mechanics under negative-pressure ventilation

作     者：Quiros, K. A. M. Nelson, T. M. Ulu, A. Dominguez, E. C. Nordgren, T. M. Eskandari, M. 

作者机构：Univ Calif Riverside Dept Mech Engn Riverside CA 92521 USA Univ Calif Riverside Riverside Sch Med Div Biomed Sci Riverside CA USA Univ Calif Riverside Environm Toxicol Grad Program Riverside CA USA Univ Calif Riverside BREATHE Ctr Sch Med Riverside CA 92521 USA Colorado State Univ Dept Environm & Radiol Hlth Sci Ft Collins CO USA Univ Calif Riverside Dept Bioengn Riverside CA 92521 USA 

出 版 物：《AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY》 (Am. J. Physiol. Lung Cell. Mol. Physiol.)

年 卷 期：2025年第328卷第3期

页      面：L443-L455页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 07[理学] 071003[理学-生理学] 

基　　金：National Aeronautics and Space Administration California Space Grant Consortium Fellowship National Science Foundation Graduate Research Fellowship Program [DGE-1840991] National Heart, Lung, and Blood Institute [R01HL158926] Opportunity to Advance Sustainability, Innovation, and Social Inclusion (OASIS) grant from the University of California Riverside Office of Technology State of California Climate Action Through Resilience Program 

主　　题：biomarkers compliance COPD negative pressure pulmonary mechanics 

摘      要：Chronic obstructive pulmonary disease (COPD) is the third leading cause of death worldwide, and the progressive nature heightens the calamity of the disease. In existing COPD studies, lung mechanics are often reported under positive-pressure ventilation (PPV) and extrapolations made from these studies pose restrictions as recent works have divulged disparate elastic and energetic results between PPV and more physiological negative-pressure ventilation (NPV) counterparts. This nonequivalence of PPV and NPV must be investigated under diseased states to augment our understanding of disease mechanics. To assess the comparability of diseased pulmonary mechanics in PPV and NPV, we pose a novel study to parse out the currently entangled contributions of ventilation mode and diseased state by analyzing murine PV curves from porcine pancreatic elastase (PPE) and hog dust extract (HDE) induced COPD models under positive and negative pressures. We find that, for PPE-exposed, under NPV, volume, compliance (C, C-start, and C-def), and hysteresis are increased in diseased states and that under PPV, only compliance (C and C-start) is increased. For HDE-exposed, under NPV, volume, compliance (C, C-inf, C-def, and K), and hysteresis are decreased, whereas, under PPV, only volume and static compliance decreased. All significant mechanical variations due to disease were observed solely at higher pressures (40 cmH(2)O) under both PPV and NPV. Our nuanced conclusions indicate the detection capabilities of multiple mechanics-based biomarkers are sensitive to the ventilation mode, where NPV exhibits more altered mechanics metrics in PPE-exposed and HDE-exposed groups compared with PPV counterparts, suggesting the resolution of biomarkers when applied under NPV research considerations may offer greater versatility. NEW & NOTEWORTHY We evaluate whether ubiquitous pressure-volume (PV) curve biomarkers depend on the ventilation mode under which they were collected (i.e., positive- or negative