Regulation of antigen presentation and interleukin 10 production in murine dendritic cells via the oxidative stimulation of cell membrane using a polycation-porphyrin-conjugate-immobilized cell culture dish

作     者：Doan, Van Thi Hong Imai, Takashi Kawazoe, Naoki Chen, Guoping Yoshitomi, Toru 

作者机构：Res Ctr Macromol & Biomat Natl Inst Mat Sci 1-1 Namiki Tsukuba Ibaraki 3050044 Japan Natl Inst Infect Dis Dept Parasitol 1-23-1 Toyama Tokyo Tokyo 1628640 Japan Antimicrobial Resistance Res Ctr Natl Inst Infect Dis 4-2-1 Aobacho Higashimurayama Tokyo 1890002 Japan Leprosy Res Ctr Natl Inst Infect Dis 4-2-1 Aobacho Higashimurayama Tokyo 1890002 Japan 

出 版 物：《ACTA BIOMATERIALIA》 (Acta Biomater.)

年 卷 期：2025年第193卷

页      面：231-241页

核心收录：

基　　金：Science and Technology Foundation Japan Society for the Pro-motion of Science [P24369] 

主　　题：Oxidative stimulation Cell membrane Lipid radical Tolerogenic dendritic cells Porphyrin Cell culture dish 

摘      要：Tolerogenic dendritic cells with professional antigen presentation via major histocompatibility complex molecules, co-stimulatory molecules (CD80/86), and interleukin 10 production have attracted significant attention as cellular therapies for autoimmune, allergic, and graft-versus-host diseases. In this study, we developed a cell culture dish equipped with polycation-porphyrin-conjugate-immobilized glass (PA-HP-G) to stimulate immature murine dendritic cell (iDCs). Upon irradiation with a red light at 635 nm toward the PA-HP-G surface, singlet oxygen was generated by the immobilized porphyrins on the PA-HP-G surface. When iDCs were cultured on the PA-HP-G surface, moderate light irradiation generated lipid radicals without excessive generation of reactive oxygen species in the cytoplasm and nucleus, which led to the oxidative stimulation of the iDC cell membrane without cell death. Light irradiation changed the morphology of dendritic cells on the PA-HP-G surface to a treelike structure with dendrites, accelerated their maturation, and enhanced the antigen-presenting ability for the ovalbumin peptide via major histocompatibility complex class I molecules. Additionally, the antigen-presenting dendritic cells on the PA-HP-G surface produced the anti-inflammatory cytokine interleukin 10 upon light irradiation. These results indicated that upon moderate light irradiation, the PA-HP-G surface regulated the maturation of iDCs into tolerogenic dendritic cells. Statement of Significance: center dot Cell culture dish is developed for selective oxidative stimulus of cell membrane. center dot 1O2 is generated from polycation/porphyrin-immobilized glass by light irradiation. center dot Lipid radicals are generated without generation of ROS in cytoplasm and nuclei. center dot Immature dendritic cells are maturated by oxidative stimulation of cell membrane. center dot Oxidative membrane stimulus enhances antigen-presentation and IL-10 secretion.