Trophic transfer of carbon-14 from algae to zebrafish leads to its blending in biomolecules and the dysregulation of metabolism via isotope effect

作     者：Shipeng Dong Renquan Deng Hang Zeng Pengfei Xue Sijie Lin Dongmei Zhou Liang Mao Shipeng Dong;Renquan Deng;Hang Zeng;Pengfei Xue;Sijie Lin;Dongmei Zhou;Liang Mao

作者机构：State Key Laboratory of Pollution Control and Resource Reuse School of the Environment Chemistry and Biomedicine Innovation Center Nanjing University College of Environmental Science & Engineering State Key Laboratory of Pollution Control and Resource Reuse Tongji University 

出 版 物：《National Science Review》 (国家科学评论(英文版))

年 卷 期：2025年第12卷第1期

页      面：155-166页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 07[理学] 

基　　金：supported by the National Natural Science Foundation of China (22125602  U2067215  22341601  22076078 and 22376095) 

主　　题：organically bound carbon-14 trophic transfer isotope effects metabolism dysregulation isotopic substitution 

摘      要：Carbon-14（C-14） has been a major contributor to the human radioactive exposure dose,as it is released into the environment from the nuclear industry in larger quantities compared to other *** most abundant nuclide enters the biosphere as organically bound C-14（OBC-14）,posing a potential threat to public ***,it remains unknown how this relatively low radiotoxic nuclide induces health risks via chemical effects,such as isotope *** establishing a trophic transfer model involving algae（Scenedesmus obliquus）,daphnia（Daphnia magna） and zebrafish（Danio rerio）,we demonstrate that rapid incorporation and transformation of inorganic C-14 by algae into OB C-14 facilitates the blending of C-14 into the biomolecules of *** find that internalized C-14 is persistently retained in the brain of zebrafish,affecting DNA methylation and causing alterations in *** isotope tracing metabolomics with C-14 exposure further reveals the involvement of C-14 in various critical metabolic pathways,including one-carbon metabolism and nucleotide *** thus characterize the kinetic isotope effects for12C/14C in the key reactions of these metabolic pathways through kinetic experiments and density functional theory computations,showing that the isotopic substitution of carbon in bio chemicals regulates metabolism by disrupting reaction ratios via isotope *** results suggest that inorganic C-14discharged by the nuclear industry can be biotransformed into OBC-14 to impact metabolism via isotope effects,providing new insights into understanding the health risk of C-14,which is traditionally considered as a low radiotoxic nuclide.