FRACTIONATION OF RNA-POLYMERASE SUBSPECIES FROM SOYBEAN HYPOCOTYLS BY ISOELECTRIC-FOCUSING IN SEPHADEX

作     者：JOHNSON, LK LIEBKE, HH OBRIEN, TJ 

作者机构：UNIV SO CALIF ALLAN HANCOCK FDN DEPT BIOL CHEM LOS ANGELES CA 90007 USA 

出 版 物：《ANALYTICAL BIOCHEMISTRY》 (分析生物化学)

年 卷 期：1976年第76卷第1期

页      面：311-320页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 0703[理学-化学] 

主　　题：染色质/酶学 指导DNA的RNA聚合酶类/分离和提纯 指导DNA的RNA聚合酶类/代谢 右旋糖酐类 等电聚焦/方法 同工酶类/分离和提纯 同工酶类/代谢 植物/酶学 黄豆 

摘      要：RNA polymerase enzymes isolated from chromatin of 6 day old soybean hypocotyls were resolved into 2 major and 1 minor species of activity by DEAE-Sephadex chromatography. A soluble form of the enzyme, isolated from the postchromatin supernatant fraction, shows a broad peak of activity when fractionated by this method. The elution characteristics and .alpha.-amanitin sensitivity data indicate the 2 major chromatin-bound activities are Class I and III enzymes, while the minor chromatin-bound activity and the soluble enzyme are representative of the Class II enzymes. Fractionation of these enzyme preparations by the new method of isoelectric focusing in Sephadex G-15 yields 5 distinct chromatin-bound and 4 soluble subspecies. The relationships of these observed activities to their parent DEAE classes are investigated, showing 2 subspecies within the Class I and III RNA polymerase enzymes, respectively, and 4 subspecies within the Class II enzymes.