版权所有:内蒙古大学图书馆 技术提供:维普资讯• 智图
内蒙古自治区呼和浩特市赛罕区大学西街235号 邮编: 010021
作者机构:UNIV SO CALIF ALLAN HANCOCK FDN DEPT BIOL CHEM LOS ANGELES CA 90007 USA
出 版 物:《ANALYTICAL BIOCHEMISTRY》 (分析生物化学)
年 卷 期:1976年第76卷第1期
页 面:311-320页
核心收录:
学科分类:0710[理学-生物学] 071010[理学-生物化学与分子生物学] 07[理学] 0703[理学-化学]
主 题:染色质/酶学 指导DNA的RNA聚合酶类/分离和提纯 指导DNA的RNA聚合酶类/代谢 右旋糖酐类 等电聚焦/方法 同工酶类/分离和提纯 同工酶类/代谢 植物/酶学 黄豆
摘 要:RNA polymerase enzymes isolated from chromatin of 6 day old soybean hypocotyls were resolved into 2 major and 1 minor species of activity by DEAE-Sephadex chromatography. A soluble form of the enzyme, isolated from the postchromatin supernatant fraction, shows a broad peak of activity when fractionated by this method. The elution characteristics and .alpha.-amanitin sensitivity data indicate the 2 major chromatin-bound activities are Class I and III enzymes, while the minor chromatin-bound activity and the soluble enzyme are representative of the Class II enzymes. Fractionation of these enzyme preparations by the new method of isoelectric focusing in Sephadex G-15 yields 5 distinct chromatin-bound and 4 soluble subspecies. The relationships of these observed activities to their parent DEAE classes are investigated, showing 2 subspecies within the Class I and III RNA polymerase enzymes, respectively, and 4 subspecies within the Class II enzymes.