New insights into the interplay between MALAT1 and miRNA-155 to unravel potential diagnostic and prognostic biomarkers of Behçet's disease

作     者：Sayed, Noha H. Shaker, Olfat G. Abd-Elmawla, Mai A. Gamal, Ahmed Fathy, Nevine 

作者机构：Cairo Univ Fac Pharm Biochem Dept Cairo 11562 Egypt Cairo Univ Fac Med Med Biochem & Mol Biol Dept Cairo 11562 Egypt Cairo Univ Fac Med Androl Sexol & STIs Dept Cairo 11562 Egypt 

出 版 物：《CLINICAL RHEUMATOLOGY》 (Clin. Rheumatol.)

年 卷 期：2025年第44卷第2期

页      面：775-787页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：Science, Technology & Innovation Funding Authority (STDF) Egyptian Knowledge Bank (EKB) 

主　　题：Beh & ccedil et's disease CD106 Disease activity IL-6 Non-coding RNA TNF alpha 

摘      要：The current study was deployed to evaluate the role of metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) and miR-155, along with the inflammatory markers, TNF alpha and IL-6, and the adhesion molecule, cluster of differentiation 106 (CD106), in Beh & ccedil;et s disease (BD) pathogenesis. The study also assessed MALAT1/miR-155 as promising diagnostic and prognostic biomarkers for BD. The current retrospective case-control study included 74 Egyptian BD patients and 50 age and sex-matched controls. Blood samples were collected, and then, serum samples were separated for further biochemical and molecular investigations. The gene expression of MALAT1 and miR-155 was measured using qRT-PCR, whereas the levels of TNF alpha, IL-6, and CD106 were estimated using ELISA technique. MALAT1 was significantly downregulated, whereas miR-155 was upregulated among BD patients, compared with control subjects. Levels of TNF alpha, IL-6, and CD106 were elevated in BD patients. Further downregulation in MALAT1 together with upregulation of miR-155 was observed in active BD patients, relative to the inactive group. Receiver-operating-characteristic analysis revealed that MALAT1 and miR-155 could discriminate BD patients from controls, on the one hand, and active from inactive BD patients, on the other hand. MALAT1 was negatively correlated with TNF alpha, IL-6, and CD106, while miR-155 was positively correlated with them. Logistic regression analyses demonstrated miR-155 as a significant independent predictor of BD susceptibility, and MALAT1 as an independent negative predictor of BD activity. For the first time, the current research enlightens the role of MALAT1 and miR-155 in BD pathogenesis via impacting IL-6/TNF-alpha/CD-106 signaling. As well, MALAT1 and miR-155 could be regarded as novel non-invasive biomarkers that may improve BD diagnosis and prognosis. Key Points center dot MALAT1/miR-155 exerts potential role in Beh & ccedil;et s *** dot MALAT1/miR-155