INVOLVEMENT OF TETRAHYDROFOLATE COFACTORS IN DENOVO PURINE RIBONUCLEOTIDE SYNTHESIS BY ADULT BRUGIA-PAHANGI AND DIROFILARIA-IMMITIS

作     者：JAFFE, JJ CHRIN, LR 

作者机构：Department of Pharmacology College of Medicine University of Vermont Burlington VT 05405 U.S.A. 

出 版 物：《MOLECULAR AND BIOCHEMICAL PARASITOLOGY》 (分子与生化寄生虫学)

年 卷 期：1981年第2卷第5-6期

页      面：259-270页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 

基　　金：NIAID NIH HHS [AI-12060] Funding Source: Medline 

主　　题：Brugia pahangi Dirofilaria immitis Folate metabolism Purine synthesis 5 10-Methyltetrahydrofolate oxidation FH 4 , tetrahydrofolic acid CH 3 FH 4 , 5-methyltetrahydrofolate TCA, trichloroacetic acid TLC, thin-layer chromatography f 10 FH 4 , 10-formyltetrahydrofolate HBSS, Hank's balanced salt solution 

摘      要：Adult B. pahangi in vitro, unlike mouse leukemia L1210 cells, converted 5-methyltetrahydrofolate (CH3FH4) directly to 5,10-methylenetetrahydrofolate and thence to other FH4 [tetrahydrofolate] cofactors. The excreted CO2 derived from CH3FH4 was due to the presence within the filariae of 10-formyltetrahydrofolate dehydrogenase (EC 1.5.1.6) which catalyzes the deformylation of 10-formyltetrahydrofolate. Adult B. pahangi and D. immitis, incubated in a purine-free medium containing [5-14C]CH3FH4 as the only form of folate, synthesized purine ribonucleotides radiolabeled at positions 2 and 8 of the purine ring. Presumably, 10-formyl[14C]FH4 donated Carbon 2 during the synthesis de novo of the purine ring and 5,10-methenyl[14C]FH4 donated Carbon 8.