Metabolome and RNA-seq reveal discrepant metabolism and secretory metabolism profile in skeletal muscle between obese and lean pigs at different ages

作     者：Liu Guo Junfei Xu Wenyue Zhou Sisi Chen Hanjing Shi Mengmeng Han Zekun Yang Yehui Duan Weijun Pang Yulong Yin Fengna Li 

作者机构：Laboratory of Animal Nutritional Physiology and Metabolic ProcessKey Laboratory of Agro-ecological Processes in Subtropical RegionInstitute of Subtropical AgricultureChinese Academy of SciencesChangsha410125China University of Chinese Academy of SciencesBeijing101408China College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing100049China Laboratory of Animal Fat Deposition and Muscle DevelopmentCollege of Animal Science and TechnologyNorthwest A&F UniversityYangling712100China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2025年第68卷第4期

页      面：1102-1117页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China(31972582) the STI 2030-Major Project(2023ZD04072) the Major Project of Changsha City(kh2401015) the Science and Technology Innovation Program of Hunan Province(2021RC4039) the Key Research and Development Program of Hunan Province(2022NK2026) the Youth Innovation Promotion Association CAS(Y202079) China Agriculture Research System of MOF and MARA(CARS-35) 

主　　题：skeletal muscle metabolome transcriptome biomarker metabolite intercellular communication lipid deposition pig model 

摘      要：Metabolites and metabolism-related gene expression profiles in skeletal muscle change dramatically under obesity,aging and metabolic *** obese and lean pigs are ideal models for metabolic ***,we compared metabolome and transcriptome of Longissimus dorsi(LD)muscle between Taoyuan black(TB,obese)and Duroc(lean)pigs at different *** defined the“window phaseof intramuscular fat(IMF)deposition in TB pig,which has significantly higher IMF than Duroc *** results displayed discrepant lipid composition and different expression genes(DEGs)enriched in lipid metabolism,and both metabolome and transcriptome analyses revealed stronger energy expenditure and more active amino acid and protein metabolism in Duroc pig.10 up-and 51 down-regulated biomarker metabolites with age-and breed-specificity were *** secretory metabolites,including organic acid(fumaric acid,succinate,malic acid,and gamma-aminobutyric acid),amino acid(L-lysine,and L-glutamic acid),lipid(2-hydroxyisovaleric acid,and L-carnitine)were demonstrated a significant correlation with IMF *** research highlights the huge difference of metabolic spectrum in skeletal muscle between obese and lean model and muscle-derived secretory metabolites might act as an ambassador of intercellular communication to regulate systematic metabolism.