Synergistic optimization strategies for the development of multienzymatic cascade system-based electrochemical biosensors with enhanced performance

作     者：Zhu, Yuzhuo Cheng, Zhaoming Wang, Xueqi Zhang, Chuanjun Li, Xuwen Wei, Yanxue Wang, Jiajia Fang, Yuxin Wang, Yi Zhang, Di 

作者机构：Tianjin Univ Tradit Chinese Med Coll Pharmaceut Engn Tradit Chinese Med Tianjin 301617 Peoples R China Tianjin Univ Tradit Chinese Med Inst Tradit Chinese Med Tianjin 301617 Peoples R China Tianjin Key Lab Intelligent & Green Pharmaceut Tra Tianjin 301617 Peoples R China State Key Lab Chinese Med Modernizat Tianjin 301617 Peoples R China Tianjin Univ Tradit Chinese Med Coll Acumox & Tuina Res Ctr Expt Acupuncture Sci Tianjin 301617 Peoples R China Natl Clin Res Ctr Chinese Med Acupuncture & Moxibu Tianjin 301617 Peoples R China 

出 版 物：《BIOSENSORS & BIOELECTRONICS》 (Biosens. Bioelectron.)

年 卷 期：2025年第274卷

页      面：117222页

核心收录：

学科分类：0710[理学-生物学] 071011[理学-生物物理学] 07[理学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 0836[工学-生物工程] 

基　　金：Integrated Traditional Chinese and Western Medicine Research Project of Tianjin Science & Technology Development Fund of Tianjin Education Commission for Higher Education [2023ZD027, 2021KJ122] Natural Science Foundation of Tianjin [22JCYBJC00990] Young Scientific and Technological Talents (Level Two) in Tianjin [QN20230231] 

主　　题：Multienzymatic system Electrochemical biosensor Cascade reaction Synergistic strategy Enzymatic biosensor 

摘      要：Multienzymatic cascade system (MCS) strategies have been a topic of growing interest in the electrochemical biosensor research field owing to their many advantages. By combining two or more enzymes in an appropriate manner, MCS approaches can extend the range of detection for particular analytes while improving the overall efficiency of biocatalytic cascade reactions. Compared with mono-enzyme biosensors, the integration of MCS and electrochemical biosensor platforms is inherently more challenging owing to the increased complexity of the resultant system. In recent years, substantial progress in the development of MCS-based electrochemical biosensors with enhanced analytical performance has been made. This review provides an overview of the types of MCS strategies and their biosensor applications, together with a summary of synergistic optimization approaches that can help improve key parameters including sensitivity, selectivity, and stability when designing MCS-based electrochemical biosensors. These discussions include examples of published biosensor platforms (2016-2024) while also surveying key advances in associated research areas including redox mediators/enzyme coimmobilization, enzyme engineering, multienzyme spatial regulation, enzyme-nanozyme integration and others. Lastly, a brief overview of current challenges and future perspectives pertaining to MCS-based electrochemical biosensor design is provided.