mTor limits autophagy to facilitate cell volume expansion and rapid wound repair in Drosophila embryos

作     者：Scepanovic, Gordana Balaghi, Negar Rothenberg, Katheryn E. Fernandez-Gonzalez, Rodrigo 

作者机构：Univ Toronto Dept Cell & Syst Biol Toronto ON M5S 3G5 Canada Univ Toronto Ted Rogers Ctr Heart Res Toronto ON M5G 1M1 Canada Univ Toronto Inst Biomed Engn Toronto ON M5S 3G9 Canada Hosp Sick Children Dev & Stem Cell Biol Program Toronto ON M5G 1X8 Canada Univ Iowa Dept Biol Iowa City IA 52242 USA 

出 版 物：《DEVELOPMENTAL CELL》 (Dev. Cell)

年 卷 期：2025年第60卷第10期

页      面：1400-1410.e3页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Ontario Graduate Scholarship Alexander Graham Bell Canada Graduate Scholarship from the Natural Sciences and Engineering Research Council of Canada (NSERC) Canadian Institutes of Health Research Ted Rogers Centre for Heart Research NSERC [418-438-13] Canada Foundation for Innovation Translational Biology and Engineering Program from the Ted Rogers Centre for Heart Research 156279 

主　　题：wound healing epithelial morphogenesis collective cell movement cell volume Drosophila embryo quantitative microscopy image analysis 

摘      要：Embryonic wounds repair rapidly, with no inflammation or scarring. Embryonic wound healing is driven by collective cell movements facilitated by the increase in the volume of the cells adjacent to the wound. The mechanistic target of rapamycin (mTor) complex 1 (TORC1) is associated with cell growth. We found that disrupting TORC1 signaling in Drosophila embryos prevented cell volume increases and slowed down wound repair. Catabolic processes, such as autophagy, can inhibit cell growth. Five-dimensional microscopy demonstrated that the number of autophagosomes decreased during wound repair, suggesting that autophagy must be tightly regulated for rapid wound healing. mTor inhibition increased autophagy, and activating autophagy prevented cell volume expansion and slowed down wound closure. Finally, reducing autophagy in embryos with disrupted TORC1 signaling rescued cell volume changes and rapid wound repair. Together, our results show that TORC1 activation upon wounding negatively regulates autophagy, allowing cells to increase their volumes to facilitate rapid wound healing.