Maize transcription factor ZmEREB167 negatively regulates starch accumulation and kernel size

作     者：Xiangyu Qing Jianrui Li Zhen Lin Wei Wang Fei Yi Jian Chen Qiujie Liu Weibin Song Jinsheng Lai Baojian Chen Haiming Zhao Zhijia Yang Xiangyu Qing;Jianrui Li;Zhen Lin;Wei Wang;Fei Yi;Jian Chen;Qiujie Liu;Weibin Song;Jinsheng Lai;Baojian Chen;Haiming Zhao;Zhijia Yang

作者机构：State Key Laboratory of Maize Bio-breedingKey Laboratory of Genome Editing Research and ApplicationMinistry of Agriculture and Rural AffairsDepartment of Plant Genetics and BreedingNational Maize Improvement CenterCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China State Key Laboratory of Crop Gene Resources and Breeding/Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Engineering Research Center of Plant Growth RegulatorCollege of Agronomy and BiotechnologyChina Agricultural UniversityBeijing 100193China Frontiers Science Center for Molecular Design BreedingBeijing 100193China Sanya Institute of China Agricultural UniversitySanyaHainan 572024China 

出 版 物：《Journal of Genetics and Genomics》 (遗传学报(英文版))

年 卷 期：2025年第52卷第3期

页      面：411-421页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by STI 2030-Major Project(2023ZD04069) National Key Research and Development Program of China(2023YFD1202900) The National Science Fund for Distinguished Young Scholars(32425041) The“Breakthrough”Science and Technology Project of Tongliao(TL2024TW001) Science and Technology Demonstration Project of Shandong Province(2024SFGC0402) Pinduoduo-China Agricultural University Research Fund(PC2023A01004) 

主　　题：Maize EREB protein BETL Starch Kernel development 

摘      要：Transcription factors play critical roles in the regulation of gene expression during maize kernel *** maize endosperm,a large storage organ,accounting for nearly 90%of the dry weight of mature kernels,serves as the primary site for starch *** this study,we identify an endosperm-specific EREB gene,ZmEREB167,which encodes a nucleus-localized EREB *** of ZmEREB167 significantly increases kernel size and weight,as well as starch and protein content,compared with the wild *** situ hybridization experiments show that ZmEREB167 is highly expressed in the BETL as well as PED regions of maize ***-luciferase assays show that ZmEREB167 exhibits transcriptionally repressor activity in maize *** analysis reveals that a large number of genes are up-regulated in the Zmereb167-C1 mutant compared with the wild type,including key genetic factors such as ZmMRP-1 and ZmMN1,as well as multiple transporters involved in maize endosperm *** of RNA-seq and ChIP-seq results identify 68 target genes modulated by *** find that ZmEREB167 directly targets OPAQUE2,ZmNRT1.1,ZmIAA12,ZmIAA19,and ZmbZIP20,repressing their *** study demonstrates that ZmEREB167 functions as a negative regulator in maize endosperm development and affects starch accumulation and kernel size.