Covalent Organic Frameworks with Fe/Co Single-Atom Nanozyme for Alendronate Sodium Detection

作     者：Cao, Jingcheng Lv, Jie Yin, Yingchao Bai, Qingqing Li, Meng Hou, Zhiyong 

作者机构：Hebei Med Univ Hosp 3 Dept Orthopaed Surg Shijiazhuang 050051 Peoples R China Hebei Med Univ Hosp 3 Orthopaed Res Inst Hebei Prov Shijiazhuang 050051 Peoples R China Hebei Med Univ Coll Pharm Key Lab Innovat Drug Dev & Evaluat Shijiazhuang 050017 Peoples R China Hebei Med Univ Postdoctoral Mobile Stn Basic Med Shijiazhuang 050017 Peoples R China 

出 版 物：《ACS APPLIED NANO MATERIALS》 (ACS Appl. Nano Mat.)

年 卷 期：2025年第8卷第3期

页      面：1490-1498页

核心收录：

基　　金：Hebei Provincial?Postdoctoral Science?Foundation [H2024206134] Key Project of Hebei Provincial Natural Science Foundation [226Z2601G] Central Guidance on Local Science and Technology Development Fund of Hebei Province [QN2023027] Science and Technology Project of Hebei Education Department [B2023003032] Postdoctoral Fund of Hebei Province [2022LCTD-B32] Support Program for Clinical Medicine Innovation Research Teams in the 14th Five-Year Plan of Hebei Medical University 

主　　题：single-atom nanozymes peroxidase-likeactivity colorimetric detection alendronate sodium Fe/Co-SA-NC nanozyme 

摘      要：Constructing nanozyme-based single atoms with exposed metal-Nx catalytic sites for alendronate sodium (ALDS) tablet quality control is a complex and uncommon task. Herein, we presented an innovative colorimetric assay to detect ALDS utilizing carbon-supported Fe/Co dual-atom nanozymes (Fe/Co-SA-NCs) derived from a precursor of porphyrin covalent organic frameworks (COFs). The Fe/Co-SA-NC nanozymes, featuring an Fe-N-5 active site and electronic interactions between Fe and Co, exhibited enhanced peroxidase-like activity. Experimental findings and theoretical calculations elucidated that the superior activity of Fe/Co-SA-NCs stemmed from the synergistic effect of the dual atoms, which boosted substrate adsorption and reduced the reaction barrier. When integrated with ALDS and Fe/Co-SA-NCs, the peroxidase-like activity of Fe/Co-SA-NCs could be inhibited due to ALDS-Fe3+ coordination. Leveraging this mechanism, a rapid and selective colorimetric method was developed for detecting ALDS. The Fe/Co-SA-NC sensor showed a detection limit of 0.96 mu M for ALDS in phosphate buffer, surpassing traditional techniques in terms of efficiency, accuracy, and simplicity. Moreover, the system was successfully utilized to control the pharmaceutical quality of ALDS and to determine ALDS in urine samples. This work opens a powerful avenue for developing efficient dual-atom nanozymes from COFs and expands the potential applications of nanozyme clinical pharmacy.